木榄提取物及单体化合物对神经细胞保护作用的研究

【作者】 周媛； 吴军； 刘朝霞；

【机构】 西安外事学院医学院药学系； 天然产物研究与利用湖北省重点实验室(三峡大学)；

【摘要】 目的研究中国海南红树植物木榄提取物及其单体化合物对神经细胞的保护作用。方法对木榄成分提取、分离结构鉴定后,用H2O2处理人神经瘤母细胞(SH-SY5Y细胞),建立细胞损伤模型,以MTT法检测木榄提取物及单体化合物的细胞增殖作用与抗氧化活性,评价其神经保护作用。结果木榄乙醇提取物、石油醚萃取部位、乙酸乙酯萃取部位及部分单体化合物能很好地对抗H2O2诱导的SH-SY5Y细胞损伤。单体化合物在低剂量(1.25-5μg·mL~1)时量效关系良好,随着浓度的增加,神经保护作用逐渐增强。结论木榄能能很好地对抗H2O2诱导的SH SY5Y细胞损伤,具有显著的神经保护作用,该作用与减少胞内氧自由基有关,其机制有待进一步研究。更多还原

【Abstract】 Objective To investigate neuroprotective effect of the Chinese Hainan mangrove plant,Bruguiera gymnorrhiza.Methods Compounds were isolated and purified with silica column chromatography,Sephadex LH-20 column chromatography and HPLC methods.Their structures were identified by physicochemical analysis,spectroscopic analysis(1H-NMR.13C-NMR and MS).After extraction and purification,H2O2 oxidative damage model to human neuroblastoma strains SH-SY5 Y cells was established)and then the cell toxicity and the protective effects under oxidative stress were evaluated by MTT method.Results The ethanol extract,petroleum ether extract and ethyl acetate extract of B.gymnorrhiza showed better antioxidant activities for SH-SYSY cells.Among the compounds oleic acid(BGP-1),physcion(BGP-3),Mansonone H(BGP-4).Catacerebroside B(BGP-5),caffeic acid(BGE-9) and rhein(BGE-10)had more significant protective effects and showed the dose-dependent effect at the low concentrations(1.25-5μg/mL).Conclusion B.gymnorrhiza can be developed as a potential drug for neurodegenerative diseases.更多还原