【作者】 张彤杰； 王建跃； 王恒；

【Author】 Zhang Tong-jie;Wang Jian-yue;Wang Heng;Daishan maternal and child health and family planning service center;Zhejiang Provincial Key Laboratory of Health Risk Factors for Seafood,Zhoushan Municipal For Disease Control and Prevention;

【机构】 岱山县高亭镇中心卫生院； 浙江省海产品健康危害因素关键技术研究重点实验室,舟山市疾病预防控制中心；

【摘要】 目的:建立尿液中睾酮和雄烯二酮的液相质谱-串联质谱检测法。方法:尿液经酶解后,使用乙酸乙酯萃取,采用Hypersil GOLD^TMaQ C18色谱柱（100×2.1 mm,1.9μm）进行分离,正离子条件下三重四级杆串联质谱多反应监测模式进行检测,内标法定量。结果:2种雄激素的相关系数均大于0.99,定量限均为0.05μg/L,平均回收率范围为74.7-112.0%,相对标准偏差（RSD%）为4.6-15.7%。结论:该方法准确可靠,可以应用于婴儿尿液中睾酮和雄烯二酮的准确测定。更多还原

【Abstract】 Objective:To develop a method for determination of testosterone and androstendione in infant urine by ultra high performance liquid chromatography- tandem mass spectrometry.Methods:The analytes were extracted with ethyl acetate after enzymatic hydrolysis.The separation was carried out on a Hypersil GOLD^TM aQ C18column（100 ×2.1 mm,1.9 μm） with methanol and water as mobile phase.The quantification was accomplished by internal standard method.Results:The calibration curves showed good linearity for both analytes with detection limits of 0.05 μg/L.The spiked recoveries in blank matrix ranged from 74.7-112.0%with RSDs less than 20%.Conclusion:The method was accurate and reliable,and was suitable for the determination of testosterone and androstendione in infant urine.更多还原