【作者】 王凤龙； 高苗； 申莉莉； 钱玉梅； 余广宏； 王耀峰； 杨金广；

【Author】 WANG Feng-long;GAO Miao;QIAN Yumei;SHEN Lili;YU Guanghong;WANG Yaofeng;YANG Jinguang;Key Laboratory of Tobacco Pest Monitoring Controlling & Integrated Management,Tobacco Research Institute,Chinese Academy of Agricultural Sciences;Baoshan Oriental Tobacco Company Ltd;Qingyang Company,Gansu Provincial Tobacco;

【机构】 烟草行业烟草病虫害监测与综合治理重点实验室/中国农业科学院烟草研究所； 云南烟草保山香料烟有限责任公司； 甘肃省烟草公司庆阳市公司；

【摘要】 由青枯雷尔氏菌(Ralstonia solanacearum)侵染引起的烟草青枯病是烟草上主要的病害之一,裂解性噬菌体(Ralstonia phagy)∈RS-1分自中国农业科学院烟草研究所即墨试验农场烟草青枯病病圃土壤,对R.solanacearum专性寄生的一类噬菌体,可侵染R.solanacearum,引起菌体的裂解,从而阻止R.solanacearum分裂和繁殖,进而控制烟草青枯病的发生流行,因此,∈RS-1具有开发新的抗菌制剂潜力。本文通过盆栽试验,预前利用∈RS-1溶液(1×1010pfu/mL)灌根,可限制抑制R.solanacearum侵染烟草根部,防止烟草青枯病的发生危害,其防治效果可达94.87%,显著高于农用链霉素的防效。利用宏基因组学方法对噬菌体处理的土壤的细菌种群多样性进行了检测分析,结果表明,表明在噬菌体处理组中土壤中R.solanacearum含量显著低于未处理组,其他微生物种群影响不显著,但在噬菌体处理的土壤中,细菌菌体数量存在降低趋势,相比较空白对照,放线菌种群丰度表现为下降趋势,因此噬菌体∈RS-1处理土壤对土壤细菌种群多样性具有一定的调控作用。更多还原

【Abstract】 Tobacco bacterial wilt caused by Ralstonia solanacearum is one of the main diseases of tobacco.Ralstonia phagy ∈RS-1 was isolated from the soil of tobacco bacterial wilt nurseries from experimental fields of the tobacco research institute of CAAS Institute, Jimo District, Qingdao City. ∈ RS-1 is obligate parasites of R.solanacearum, can infect and lyse R.solanacearum, which preventing them from dividing and reproducing, then control the disease pandemic. So ∈RS-1 has real potential as a new microbial inoculants on R.solanacearum. We irrigated the tobacco root by the ∈ RS-1 culture( 1×1010pfu/mL) before artificial inoculation the R.solanacearum. The result showed that a significant decrease in disease severity was observed when coinoculated with ∈ RS-1. The control efficiency of ∈ RS-1 against R.solanacearum can reach 94.87%, and was higher than control by agricultural streptomycin. The diversity of bacterial community in soil of treated by ∈RS-1 was studied using metagenomic technique. The result showed that the soil R.solanacearum content is obviously lower than contrast, and do not have remarkable influence to the other microorganisms. But in the soil treated by ∈ RS-1, the quantity of bacteria showed a descending trend,The quantity of actinomyces showed decreased trend compared to the blank control. So ∈ RS-1 has the role of adjust the diversity of soil bacterium.更多还原