Ascaridoid parasites infecting in the starry batfish Halieutaea stellata(Vahl)(Lophiiformes:Ogcocephalidae) from the East and South China Sea

【作者】 李亮； Wen-Ting Zhao； Yan-Ning Guo； Lu-Ping Zhang；

【Author】 Liang Li;Wen-Ting Zhao;Yan-Ning Guo;Lu-Ping Zhang;Key Laboratory of Animal Physiology,Biochemistry and Molecular Biology of Hebei Province,College of Life Science,Hebei Normal University;

【机构】 Key Laboratory of Animal Physiology,Biochemistry and Molecular Biology of Hebei Province,College of Life Science,Hebei Normal University；

【摘要】 The starry batfish Halieutaea stellata(Vahl) is a small,benthic fish found in Indo-West Pacific Oceans.However,our present knowledge of the helminth parasites of this fish is still fragmentary.In the present study,a total of 29 fish collected from the East and South China Sea,were examined to determine the prevalence,intensity,and species composition of nematode parasites in H.stellata.Using morphological and molecular approaches,four species of ascaridoid nematodes were found parasitic in this fish host,including the adults and fourth-stage larvae of Raphidascaroides nipponensis Yamaguti,1941,adults and third-stage larvae of Raphidascaris lophii(Wu,1949),third- and fourth-stage larvae of Hysterothylacium larval typeⅣ-A of Shamsi,Gasser & Beveridge,2013 and third-stage larvae of Hysterothylacium amoyense(Hs(u|¨),1933).Halieutaea stellata represents a new host record for the three last-named nematode.Raphidascaroides nipponensis with the highest prevalence(82.5%) and intensity(mean=13.5) of infection,was considered as the dominant parasite species in H.stellata.The detailed morphology of the different developmental stages of the four nematode species was studied using light and scanning electron microscopy.All nematode species were also genetically characterized by sequencing and analysing the internal transcribed spacer(ITS) of the ribosomal DNA.The present study provides further data on the occurrence of nematode parasites in H.stellata and also contributes to facilitate an accurate and rapid diagnosis of the infection by these little-known nematodes.更多还原

【Abstract】 The starry batfish Halieutaea stellata(Vahl) is a small,benthic fish found in Indo-West Pacific Oceans.However,our present knowledge of the helminth parasites of this fish is still fragmentary.In the present study,a total of 29 fish collected from the East and South China Sea,were examined to determine the prevalence,intensity,and species composition of nematode parasites in H.stellata.Using morphological and molecular approaches,four species of ascaridoid nematodes were found parasitic in this fish host,including the adults and fourth-stage larvae of Raphidascaroides nipponensis Yamaguti,1941,adults and third-stage larvae of Raphidascaris lophii(Wu,1949),third- and fourth-stage larvae of Hysterothylacium larval typeⅣ-A of Shamsi,Gasser & Beveridge,2013 and third-stage larvae of Hysterothylacium amoyense(Hs(u|¨),1933).Halieutaea stellata represents a new host record for the three last-named nematode.Raphidascaroides nipponensis with the highest prevalence(82.5%) and intensity(mean=13.5) of infection,was considered as the dominant parasite species in H.stellata.The detailed morphology of the different developmental stages of the four nematode species was studied using light and scanning electron microscopy.All nematode species were also genetically characterized by sequencing and analysing the internal transcribed spacer(ITS) of the ribosomal DNA.The present study provides further data on the occurrence of nematode parasites in H.stellata and also contributes to facilitate an accurate and rapid diagnosis of the infection by these little-known nematodes.更多还原