【作者】 秦青松； 孙聆东； 严纯华；

【Author】 Qing-Song Qin;Ling-Dong Sun;Chun-Hua Yan;Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, PKU-HKU Joint Laboratory in Rare Earth Materials and Bioinorganic Chemistry, College of Chemistry and Molecular Engineering, Peking University;

【机构】 北京分子科学国家实验室稀土材料化学及应用国家重点实验室北京大学-香港大学稀土材料和生物无机化学联合实验室北京大学化学与分子工程学院；

【摘要】 荧光探针被广泛应用于光学成像及生物诊疗中[1]。与可见光相比,近红外光(700-1600 nm)受到生物组织的吸收和散射作用更小,可实现深层组织的光学成像,因此发展近红外发光探针具有重要意义。稀土掺杂的近红外发光纳米颗粒具有优异的光学性质,比如大的斯托克斯位移,良好的光稳定性以及低毒性。我们利用热分解方法合成了钕离子掺杂的稀土氟化物复盐纳米颗粒,在808 nm激光激发下,纳米颗粒表现出860 nm,1060 nm和1350 nm的近红外发光。进一步,我们通过外延生长的方式得到了核壳结构的纳米颗粒,有效增强了纳米颗粒的近红外发光。在140 m W/cm~2的激发功率下,纳米颗粒的发光可有效地穿透厚度为20 mm的猪肉组织。通过尾静脉注射,可以清晰地观察到裸鼠血管图像。这些结果表明我们发展的近红外发光纳米颗粒在生物光学成像中具有很大的应用潜力。更多还原

【Abstract】 The fluorescent probe working in the NIR(700-1600 nm) region has the advantages of high sensitivity, high resolution, deep tissue penetration and low auto-fluorescence, which can enable real-time and noninvasive imaging. Herein, high-quality Nd3+ doped fluoride nanoparticles(NPs) were synthesized with a modified thermal decomposition method. Transmission electron microscope(TEM) images show that the as-synthesized NPs were uniform in size and morphology. Upon 808 nm NIR laser irradiation, the as-synthesized NPs show sharp and intense NIR emission. The hydrophobic NPs were transferred to the aqueous phase by modifying FDA-approved polyethylene glycol(PEG). For the hydrophilic NPs, the penetration depth of 2 cm through phantom tissue could be achieved with the power density of 140 m W/cm2. Moreover, we observed the clear blood vessel imaging by intravenously injecting NPs@PEG into nude mice. Those results demonstrate the nanomaterials have great potential in bio-imaging.更多还原