【作者】 刘莉； 李如燕； 赵健； 许媛媛； 王兆寅； 戴志晖；

【Author】 Li Liu;Ruyan Li;Jian Zhao;Yuanyuan Xu;Zhaoyin Wang;Zhihui Dai;Jiangsu Key Laboratory of Biofunctional Materials,College of Chemistry and Materials Science,Nanjing Normal University;Key Laboratory of Animal Physiology and Biochemistry,College of Veterinary Medicine,Nanjing Agricultural University;

【机构】 南京师范大学化学与材料科学学院江苏生物功能材料重点实验室； 南京农业大学动物医学学院动物生理学和生物化学重点实验室；

【摘要】 这项工作巧妙设计了一种稳定且可重复使用的电化学传感器无标记检测多聚腺苷二磷酸核糖聚合酶(PARP)及其抑制剂。其中,组装在电极表面的G四联体成功束缚并激活PARP产生带负电荷的多聚腺苷二磷酸核糖合成酶(PAR)。带负电荷的PAR可以吸附带正电的信号分子,因此可以实现无标记定量检测PARP及其抑制剂。这种分析策略避免了修饰基底或抗体的参与,大大简化了实验过程。同时,修饰上的DNA具有良好的稳定性,G四联体和PARP之间的相互作用可以轻易地消除。因此,提出的生物传感器具有稳定性和可重复利用性,这在相关的生物传感器中是很重要的。更多还原

【Abstract】 A stable and reusable electrochemical biosensor for label-free detection of poly(ADP-ribose)polymerase(PARP) and its inhibitor is ingeniously designed.In this work,G-quadruplex is assembled on the electrode surface,which successfully tethers and activates PARP generating the negative-charged poly(ADP-ribose) polymer(PAR).Owing to the natural property of negative charge,PAR can absorb positive-charged electrochemical signals molecules,thus,the label-free quantification of PARP and its inhibitor can be simply implemented.The analytical strategy gets rid of the participation of modified substrates or antibodies,greatly facilitating the procedure.Meanwhile,due to the good stability of the modified DNA and the uncomplicated elimination of the interaction between G-quadruplex and PARP,the proposed biosensor is endowed with the characteristics of stability and reusability,which is desirable in related biosensors.更多还原