【作者】 周小明； 廖玉辉； 邢达；

【Author】 Xiaoming Zhou;Yuhui Liao;Da Xing;MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science,College of Biophotonics,South China Normal University;

【机构】 华南师范大学生物光子学研究院,激光生命科学教育部重点实验室；

【摘要】 RNA未经保护而暴露,通常会在几个小时内发生不可逆的降解。RNA的这种不稳定性主要是由于自然界普遍存在的RNA酶污染。目前,利用RNA酶抑制剂和提取过程中使用TRIzol RNA试剂进行RNA保护是金标准方法。但是,这些方法只提供被动的且非连续的RNA保护。本研究中,我们展示RNA酶与RNA均能与石墨烯(GO)发生强吸附效应,这种共吸附的效应促使我们发展一种新的基于屏蔽效应的RNA稳定方法。石墨烯屏蔽效应可以稳定未经任何处理的RNA长达四天,或确保RNA能抵抗高浓度的核糖核酸酶(40单位)的降解。此外,我们进一步发展了基于了石墨烯屏蔽效应的RNA提取法,该方法可以快速获得高质量的RNA。石墨烯的这种盾效应有望成为RNA稳定和提取的一种新的换代技术。更多还原

【Abstract】 When RNA is exposed in air,irreversible degradation appears within hours.This instability of RNA is mainly attributed to the ubiquitous RNase contamination.Currently,RNA-protection using RNase inhibitor and RNA-extraction with TRIzol are the gold standards.But these approaches only provide RNA with a passive and inconsecutive protection.Herein,we demonstrate a novel RNA-protection assay based on graphene oxide(GO) shield that activated by the joint contributions from immobilization of RNase and RNA.GO shield can protect the RNA in air for 4 days or allow RNA to resist the degradation with high-concentration RNase(40 Unit).Furthermore,the protective RNA-extraction assay based on GO shield is constructed to acquired high-quality RNA.This assay realizes the continuous protection of RNA,it may become a new paradigm of RNA-extraction.Hence,this discovery of a new GO function reveals that GO shield can act as the effective RNA stabilizing agent which possesses potentials to revolutionize the field of RNA protection and extraction.更多还原