【作者】 李娟； 洪诚毅； 吴淑贤； 梁虹； 杨黄浩；

【Author】 Juan Li;Cheng-Yi Hong;Shu-Xian Wu;Hong Liang;Huang-Hao Yang;The Key Lab of Analysis and Detection Technology for Food Safety of the MOECoUege of Chemistry,Fuzhou University;

【机构】 福州大学食品安全分析与检测技术教育部重点实验室化学学院；

【摘要】 疏水性纳米成像探针具有发光效率高、发射峰半峰宽窄、光稳定性好等优点,但其疏水性使其难以应用于生物成像分析和生物医学领域。本工作利用DNA四面体纳米结构对疏水性纳米粒子进行转相,并同时实现对纳米粒子的表面功能化。DNA四面体由3条修饰羧基的单链DNA和1条包含核酸适配体序列的单链DNA组装而成。通过DNA四面体表面修饰,提高了纳米粒子在水相中的分散性、稳定性和生物相容性,且表面DNA保留其生物功能。核酸适配体保留原有的肿瘤靶向性能,提高纳米粒子的细胞摄取量。DNA四面体转相的上转换荧光纳米粒子可实现肿瘤靶向的活细胞荧光成像。该方法具有通用性,可用于其它多种疏水性纳米粒子,如用于Fe₃O₄磁性纳米粒子的转相和表面修饰,实现癌细胞的靶向磁共振成像。该方法有望进一步扩展疏水性纳米粒子在生物医学中的应用^[1]。更多还原

【Abstract】 Hydrophobic nanoparticles have advantages of narrow fluorescence spectrum,high luminous efficiency,high stability,etc.However,they are limited in bioimaging and biomedical applications due to their hydrophobicity.This work reports a facile strategy for phase-transfer and surface biofunctionalization of hydrophobic nanomaterials using aptamer-pendant DNA tetrahedron nanostructures.The Janus DNA tetrahedron nanostructures are constructed by three carboxyl group-modified DNA strands and one aptamer sequence.After surface biofunctionalization by DNA tetrahedron,the nanoparticles have improved dispersion,stability,and biocompatibility in aqueous phase,and DNAs retain their biological function.The incorporation of the aptamers adds targeting ability and enhances intracellular uptake.DNA tetrahedron modified upconversion nanoparticles(UCNPs) can be successfully used for targeted fluorescence imaging in living cells.This method is universal.DNA tetrahedron modified iron oxide nanoparticles(IONPs) can be considered as a potential candidate for targeted MRI.This method may further expand biomedical applications of hydrophobic nanomaterials.更多还原