【作者】 吴永祥； 张代亮； 冯莉莉； 李俊彬； 张晓兵； 谭蔚泓；

【Author】 Yong-Xiang Wu;Dai-Liang Zhang;Li-Li Feng;Jun-Bin Li;Xiao-Bing Zhang;Weihong Tan;Molecular Sciences and Biomedicine Laboratory(MBL),State Key Laboratory for Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering and College of Biology,Collaborative Innovation Center for Molecular Engineering and Theranostics,Hunan University;

【机构】 分子科学与生物医学实验室化学计量与化学生物传感国家重点实验室湖南大学化学化工学院湖南大学生物学院湖南大学化学与分子医学协同创新中心；

【摘要】 激发态分子内质子转移(ESIPT)反应是质子转移反应的一种,由于其在化学和生物中的应用而被广泛的关注。半胱氨酸(cysteine,Cys)是生物体内的一种非常重要的氨基酸,它能调控生物体内的催化性能,还能够对外源性氧化物质起到生化解毒的作用。相对于常见的检测半胱氨酸的方法,荧光检测法由于其操作简单、高选择性、高灵敏度、不损伤待测样品本身以及易于实时监测等优点,因而引起了科学家的广泛关注。传统的荧光探针在检测时是溶液的状态,很容易流动,因此会造成检测结果的不稳定。因此,在本工作中我们合成了2-(2-羟基苯基)-4(3H)-喹唑酮(HPQ)的衍生物MA-HPQ,它具有两个特点:1.激发态分子内质子转移;2.溶液状态是无荧光的,固态发出荧光,这样就可以获得较高的灵敏度,同时避免传统荧光探针流动相的缺点,获得可靠的检测结果。基于以上的优点,我们设计并合成了基于激发态分子内质子转移的固态发光探针用于细胞内半胱氨酸的检测。其实验结果是令人满意的。更多还原

【Abstract】 ESIPT is a kind of proton transfer reaction,which is widely concerned because of its application in chemistry and biology.Cysteine is a very important amino acid in the body.Compared with the common cysteine detection method,fluorescence detection method caused the wide attention of scientists.The traditional fluorescent probe is in the state of the solution,it is easy to flow,so it will result in the instability of the detection results.Therefore,in the present work,we synthesized 2-(2-hydroxy phenyl)-(3H)-quinazolinone derivative MA-HPQ,it has two characteristics:1.excited state intramolecular proton transfer;2.the solution state is non fluorescent,solid-state can emit fluorescence,so that we can obtain higher sensitivity,and obtain reliable results.Based on the above advantages,we have designed and synthesized a solid-state fluorescent probe based on excited state intramolecular proton transfer for the detection of intracellular cysteine.The experimental results are satisfactory.更多还原