【作者】 黄齐林； 王亚； 徐志爱； 金利通； 张文；

【Author】 Qilin Huang;Ya Wang;Zhiai Xu;Litong Jin;Wen Zhang;College of Chemistry and Molecular Engineering,East China Normal University;Department of Chemistry,YuXi Normal Univeristy;

【机构】 华东师范大学化学与分子工程学院； 玉溪师范学院化学系；

【摘要】 本文中制备的具有可见光活性的AChE/Ag/N-F-TNs光电化学生物传感器具有宽的线性范围和较高的灵敏度,可适用于对生物样品中AChE活力（Enzyme activity,EA）的测定。实验结果表明,光电流响应与AChE活力在10 U/L²000 U/L范围内呈良好的线性关系。光生电流增加值与酶活力的线性方程为:y=5.72×10^-2x+15.95,相关系数为99.76%,检测限为2 U/L（图1 A）。将该光电化学酶生物传感器用于检测小鼠血清中AChE活力,能够取得满意的结果（图1 B）。所制备的光电化学酶生物传感器灵敏度高,响应快速。为研究AChE等重要靶向分子的活性及其变化提供了新的检测方法。更多还原

【Abstract】 In this paper,the AChE activity in rat serum was determined by photoelectrochemical analysis(PECA)method with the visible-light-actived photoelectrochemical biosensor prepared in section one.This method showed an excellent analytical performance,which exhibited a wide dynamic response range of AChE activity from 10 U/L to 2000 U/L and with a detection limit of 2 U/L.The results demonstrate that the developed PECA method offered a new approach for AChE activity study,which is related to the pathogenesis mechanism.Overall,this propsed established PECA method was very promising and could be easily extended to measure the other enzyme activity.更多还原