【作者】 王宁宁； 李继山；

【Author】 Ningning Wang;Jishan Li;State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan University;

【机构】 化学生物传感与计量学国家重点实验室湖南大学化学化工学院；

【摘要】 一氧化氮(NO)是一种气体信使分子,其在心脑血管调节、神经、免疫调节等方面有着十分重要的生物学作用,因此对一氧化氮的生物检测具有重要的意义。本文中,利用上转换纳米颗粒作为激发光源,罗丹明衍生物作为一氧化氮识别单元,采用介孔硅包覆合成技术,β-环糊精作为堵孔,构建了一种核壳型结构的上转换荧光纳米探针,并通过NO对上转换纳米颗粒与染料分子之间FRET过程的调节实现了一氧化氮的生物检测及成像。介孔硅和β-环糊精的引入,使纳米探针具有良好的生物相容性,比率型输出信号降低了背景干扰、提高了信号的重现性。在0-0.18 mM的一氧化氮PB缓冲溶液中,探针响应与NO浓度呈现良好的线性关系。更多还原

【Abstract】 NO,a messenger molecule,plays an important role in regulation of various physiological and pathophysiological processes.At high concentration,NO can cause cytotoxic.Therefore,monitoring and imaging of NO in biological surrounding is of great importance.As anti-stokes luminescence material,upconversion nanoparticle has exhibited some outstanding advantages in determination and bioimaging in organism.In this study,we synthesized a core-shell UCL nanoprobe by adsorbing RP into the mesopores of mSiO₂ shell of upconversionnanoparticles for imaging of the intracellular NO by the change of the FRET process.Using the ratio of fluorescence intensity as output signal,which provides a built-in correction for environmental effects,NO concentration is found in the range of 0 to 0.18 mM in phosphate buffer.更多还原