【作者】 赵玉婷； 杜丹； 林跃河；

【Author】 Yuting Zhao;Dan Du;Yuehe Lin;Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University;

【机构】 华中师范大学农药与化学生物学教育部重点实验室；

【摘要】 简单、准确、低成本的现场检测体系在个人医疗保健和疾病诊断上有迫切需求。但目前被公众熟知的便携式生物传感设备却只有早孕检测试纸条、血糖仪（GM）等几种。因此,为了扩宽血糖仪的应用,我们改进血糖仪,使它用于检测除了葡萄糖以外的目标物--生物标志物¹。然而大多数手持设备的灵敏度都有待提高,而在放大信号方面,由于脂质体水溶性且大的内腔容易包埋大量信号分子使其成为最有潜力的候选。因此,基于血糖仪和脂质体的显著优点,我们发展了一种便携式、低成本、易于实施的能定量检测一系列生物标志物的方法²。在这里,我们将肿瘤抑制蛋白phospho-p53¹⁵的含量定量的转化为包埋在脂质体中葡萄糖分子的含量,然后直接用GM来定量检测释放出的葡萄糖。在这里,由于脂质体中包埋数以万计的葡萄糖分子,可以显著放大检测信号,实现了高灵敏性;只要我们更换相应的抗体,便可实现一系列疾病标志物的检测,因此应用非常广泛;此外,应用商品化的血糖仪来检测,实现了便携式与低成本。更多还原

【Abstract】 A new technology was developed to quantitatively detect a broad range of disease biomarkers and proven to be portable, economical, and conveniently accessible. Measurements were performed based on releasing encapsulated glucose from antibody-tagged liposomes and subsequently detecting there leased glucose using a commercial personal glucosemeter(GM). The innovative aspect of this approach lies in the quantification of target biomarkers through the detection of glucose, thus expanding the applicability of the GM by broadening the range of target biomarkers instead of detecting only one analyte, glucose. This approach was demonstrated to be a universal technology that can be easily produced to quantify a wide variety of biomarkers in medical diagnostics, food safety, public health, and environmental monitoring.更多还原