【作者】 黄臻臻； 王皓南； 杨文胜；

【Author】 Zhenzhen Huang;Haonan Wang;Wensheng Yang;College of Chemistry, Jilin University;

【机构】 吉林大学化学学院；

【摘要】 准确、快速的定量检测人血清白蛋白在临床诊断、疾病监测和生化实验中具有重要的实际意义[1]。在诸多人血清白蛋白的分析检测方法中,基于有机分子探针的荧光检测法以高灵敏度、高选择性及操作简便等优点而备受关注。然而,有机探针分子的合成过程通常较为繁琐耗时,且需要使用有毒的有机试剂[2]。本文中,我们采用以谷胱甘肽为配体合成的荧光金纳米簇(GSH-AuNCs)代替传统的有机分子作为探针,发展了一种检测HSA的分析方法。研究发现,HSA与GSH-AuNCs之间的疏水相互作用可显著增强AuNCs的荧光发射强度。在10-550 nM范围内,荧光增强的程度与HSA的浓度呈线性关系。该方法操作简便、灵敏度高(检测限可达1.5 nM),选择性好,并且可用于生物样品中HSA的检测。更多还原

【Abstract】 Human Serum albumin(HSA) is one of the most important proteins in biomedicine and biochemistry. A number of methods have been developed for detection of HSA and the fluorescent sensors based on organic molecular probes, are of particular interest due to their high selectivity and sensitivity. However, syntheses of the organic fluorescent probes often suffer from multi-step reactions, low yields and the use of toxic reagents. Here, glutathione-capped gold nanoclusters(GSH-AuNCs) as a promising alternative to conventional organic probes were used to develop a fluorescent sensor for HSA detection. The method relies on the fact that the fluorescence intensity of GSH-AuNCs can be significantly enhanced in the presence of HSA. The present method showed good sensitivity and selectivity, and was easy to implement for quantitative detection of HSA.更多还原