【作者】 陈卓； 邹玉秀； 丁玎； 徐逸婷；

【Author】 Zhuo Chen;Yu-Xiu Zou;Ding Ding;Yi-Ting Xu;Sate Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University;

【机构】 湖南大学化学生物传感与计量学国家重点实验室,化学化工学院；

【摘要】 拉曼光谱技术作为一种振动光谱技术,已成为分子医学研究领域中有价值的检测手段,基于拉曼振动光谱的生物检测技术已被广泛的建立和发展起来。我们提出了利用碳纳米材料作为新型拉曼探针来进行生化分析研究的策略。通过将贵金属纳米粒子包裹在石墨烯薄层中,我们设计构造了贵金属石墨纳米囊拉曼生化分析平台。这种纳米囊状结构的设计既利用了石墨烯纳米材料优异的理化性能,又保证了贵金属稳定的表面等离激元性能。我们研制了超级稳定的银纳米颗粒,并通过功能化修饰核酸适配体等分子,实现了对肿瘤细胞的靶向、低背景、快速拉曼成像。我们还研制了金石墨纳米囊复合纳米结构,结合拉曼与双光子荧光成像等模式,实现了对细胞的多模态检测与成像,进一步提高了成像的准确性。此外,我们还研究了石墨纳米囊材料的生物相容性,并探讨了其在有无靶向核酸分子修饰下被细胞内吞摄取的途径,为进一步生物医学应用做了有益的探索。更多还原

【Abstract】 Raman spectroscopy is a widely used analytical method capable of providing valuable information about the chemical structure and composition of molecules. Graphitic nanomaterials possess distinctive Raman signatures,which make them ideal for sensitive Raman detection and imaging. Noble metal graphitic nanocapsules have been designed and utilized for different biomedical applications. We fabricated corrosion-resistant, water-soluble, and graphene-protected Ag Cu nanoparticles(ACG). Such stable ACG have been utilized for cell labelling, rapid Raman imaging and SERS detection. We also developed a graphene-isolated-Au-nanocrystal(GIAN) nanostructures. Multimodal cell imaging and NIR photothermal-enhanced chemotherapy have been realized with such GIAN platform. We further explored the cellular uptake mechanism of the GIAN by monitoring the intrinsic Raman and two-photon luminescence signals of GIANs. The mechanism of cellular uptake in these GIAN nanostructures is determined by the presence or absence of aptamer modification.更多还原