【作者】 李念露； 贾丽萍； 马荣娜； 贾文丽； 路以洋； 石珊珊； 王怀生；

【Author】 Nian-Lu Li;Li-Ping Jia;Rong-Na Ma;Wen-Li Jia;Yi-Yang Lu;Shan-Shan Shi;Huai-Sheng Wang;Department of Chemistry, Liaocheng University;

【机构】 聊城大学化学化工学院；

【摘要】 成功制备了一种基于碳量子点（CQDs）与Ru（bpy）₃²⁺共反应测定癌坯抗原（CEA）的夹心免疫电化学发光（ECL）传感器。实验中,将CQDs固定在聚乙烯亚胺功能化石墨烯（PEI-GO）上,然后选用CQDs-PEI-GO复合物来固定一抗Ab₁。用介孔硅（MSN）负载联吡啶钌(Ru（bpy）₃²⁺)和固定金纳米颗粒（AuNPs）,其中AuNPs能够增加电子的传导进而增大ECL信号。同时,将二抗Ab₂固定在MSN上。实验中,CQDs作为Ru（bpy）₃²⁺发光的共反应剂。在最佳实验条件下,该传感器的线性范围为1 pg·m L^-1-1000 ng·m L^-1,检出限是0.33 pg m L^-1（S/N=3）。该传感器具有良好的特异性、稳定性和重现性。更多还原

【Abstract】 A novel sandwiched electrochemiluminescence（ECL） immunosensor for the detection of carcinoembryonic antigen（CEA） based on the coreaction between carbon quantum dots and Ru（bpy）₃²⁺ was developed. Carbon quantum dots（CQDs） were assembled onto the surface of the poly（ethylenimine） functionalized graphene oxide（PEI-GO） and the complex of PEI-GO-CQDs was used to immobilize primary antibody（Ab₁）. Mesoporous silicon（MSN） was utilized to load Ru（bpy）₃²⁺ served as chemiluminescence signal and Au nanoparticles which can accelerate the electron transport to amplify the ECL signals. At the same time, the functionalized MSN was employed to label secondary antibody（Ab₂）. In this work, CQDs acted as the co-reactants of the anodic ECL of Ru（bpy）₃²⁺. Under the optimized conditions, the proposed ECL immunosensor showed a linear response to CEA with a range from 1 pg·m L^-1 to 1000 ng·m L^-1 and a detection limit of 0.33 pg m L-1（S/N=3）. In addition, the proposed immunosensor exhibited good selectivity, acceptable stability and reproducibility.更多还原