【作者】 姜晖； 王雪梅；

【Author】 Hui Jiang;Xuemei Wang;State Key Laboratory of Bioelectronics, Southeast University;

【机构】 东南大学生物电子学国家重点实验室；

【摘要】 近年来,金属纳米簇凭借其优良的光学性能和生物相容性受到越来越多的重视1。我们以胞苷及其核苷酸为模板,通过pH和前驱物比例的调节,可制备荧光发射为470 nm(蓝)、505 nm(绿)和560 nm(黄)的金纳米簇2a;进一步发现mM浓度的硫醇化合物可诱导绿色金纳米簇发生3倍的荧光增强,而其他类似结构的纳米簇均无此现象。质谱研究认为原有的较大纳米簇在此过程中裂解为更小的发光纳米簇,发光效率提高。该现象可被用于谷胱甘肽还原酶活性检测及其抑制剂的筛选2b;我们以谷胱甘肽为模板制备了弱发光的金纳米簇,证实m M浓度的硫醇化合物对该纳米簇具有5~7倍增强响应,适合细胞内GSH等成像2c。质谱结果显示硫醇化合物能有效吸附到弱发光金纳米簇上诱导荧光增强。这些工作表明金纳米簇凭借独特的光学和化学特性,在生物检测和成像方面有潜在的应用前景。更多还原

【Abstract】 Au nanoclusters(NCs) have attracted great interest due to the excellent optical properties and biocompatibility. We prepared multicolor cytidine/ its nucleotides capped Au NCs and demonstrated ~3-fold fluorescent(FL) enhancement induced by thiols at mM level, allowing the sensitive detection of glutathione reductase as well as its inhibitor screening. We also verified that 5~7 fold FL enhancement occurred when low-FL glutathione capped Au NCs were treated with thiols at μM level, allowing intracellular thiols imaging. These works indicated the great prospects of Au NCs in biomedical applications.更多还原