【作者】 龙彧茵； 周翠松； 王从敏； 尹翠云； 肖丹；

【Author】 Yuyin Long;Cuisong Zhou;Congmin Wang;Cuiyun Yin;Dan Xiao;College of chemistry, Sichuan University;

【机构】 四川大学化学学院；

【摘要】 将静电纺丝与各种信号放大技术相联用,可达到光、电等信号的转换和放大的目的。近年来,我们小组结合静电纺丝与信号放大技术,构建了高灵敏、高特异性的生物传感器。通过静电纺丝一步法制备了铱掺杂的发光纤维膜,结合紫外辐照和化学共价修饰技术,构建了葡萄糖氧化酶(GOx)功能化的荧光增强传感界面[1]。该传感界面能与溶解氧之间迅速地电子转移,实现了对葡萄糖的灵敏、快速检测,检出限低至0.1 n M,响应时间为1 s。将静电纺丝与等离子体、生物素—亲和素及核酸信号放大技术联用,发展了一种多重放大分子识别纳米平台的制备方法[2]。该平台集发夹自组装催化(CHA)放大反应、GOx催化作用和DNAzyme催化显色三种反应于纤维膜表面,实现了对HIV DNA标志物的超灵敏可视化检测,检出限低至4.8 p M,并且能特异性识别血液复杂样品中超低浓度的靶分子单碱基错配(5.0 n M)。研究结果表明,静电纺丝与信号放大技术的联用,对实现复杂样品中高灵敏、高特异性检测具有潜在优势,有望在疾病诊断、病毒检测、新药研发和筛选等领域发挥重要作用。更多还原

【Abstract】 Combining the electrospinning technology, various amplifications of optical and electric signals can be achieved. One example is the iridium complex-doped optical fibrous membrane functionalized with glucose oxidases（GOx）, which was used as a quick and sensitive fluorescence biosensor for the detection glucose.^[1] A facile and label-free multi-amplification nanoplatform was developed for the ultrasensitive visual detection of HIV DNA biomarkers.^[2] The platform combines catalytic hairpin assembly amplification, GOx biocatalysis, and DNAzyme-catalyzed colorimetric reaction together on the nanofibrous surface. The combination of electrospinning and signal amplification has great advantages of highly sensitive and specific detection in complex biological system, which would have potential applications in the field of disease diagnosis, virus detection, and drug screening.更多还原