【作者】 吴昊； 尹成日；

【Author】 Hao Wu;Chengri Yin;Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules,Ministry of Education, Yanbian University;

【机构】 吉林省延吉市延边大学长白山生物资源教育部重点实验室；

【摘要】 自然界中存在的元素硒对提高人体免疫力、抗癌防癌有着重要作用^[1]。人体缺硒或者过量补充都会导致疾病,所以对硒的分析,在环境科学、医学、生命科学有着重要的意义^[2]。本文用荧光法研究了硝酸介质中痕量硒（Ⅳ）催化溴酸钾氧化罗丹明B褪色的反应和动力学条件,建立了动力学荧光法测定硒的新方法。结果表明:在加入硝酸体积为1 mL,罗丹明B体积为1.5 mL,溴酸钾体积1.5m L,反应时间84℃和反应时间17min时,在测定范围0-0.400μg/L,线性相关系数可达到0.9973。更多还原

【Abstract】 The element selenium in nature plays an important role in improving human immuity and anti-cancer^[1]. Lacking of selenium or suppling excessly would lead to disase^[2]. Thus, the analysis of selenium is very important in environmental science, medical science and life science. In this study, a new method of kinetics fluorescence spectrophotometry for determination of trace Se（Ⅳ） is established. The results showed the optimal conditions were 1.0 mL of nitric acid, 1.5 mL of RBD, 1.5 mL of KBrO₃, 17 min of reaction time and 84℃. The analytical range of the measurement is 0 to 4.0 μg/L. Under this condition, the linearly dependent coefficient is 0.9973.更多还原