【作者】 窦海洋； 王静； 李月秋； 张晶； 丁良； 申世刚；

【Author】 Haiyang Dou;Jing Wang;Yueqiu Li;Jing Zhang;Liang Ding;Shigang Shen;School of Medicine, Hebei University;College of Chemistry and Environmental Science, Hebei University;

【机构】 河北大学医学院； 河北大学化学与环境科学学院；

【摘要】 非对称场流分离技术（AF4）是一种基于样品与外力场相互作用机理的分离技术[1]。不同于高效液相色谱,AF4池道中没有固定相和填充材料,这决定了AF4具有广泛的检测范围(10³-10¹⁸g/mol),并适用于剪切力敏感的生物样品的分离。AF4池道的独特设计使分析载液具有广泛的选择性,允许对样品进行原位分析,收集的AF4样品片段可用于其他分析手段的离线或在线分析。另外,根据AF4理论[2,3],AF4与一些检测器串联可以同时提供样品的粒径分布图。本项目通过AF4与多角光散射检测器（MALS）、示差折光检测器串联研究了发芽时间对大麦中淀粉物化性质的影响。通过体内体外实验研究了发芽大麦中淀粉物化性质和抗糖尿之间的相关性。结果发现,经过简单的发芽处理,淀粉表观密度和抗糖尿活性有所增加。另外,本项目通过AF4与紫外可见检测器（UV/VIS）、MALS和荧光检测器串联分离表征了鸡蛋蛋黄中的脂蛋白。结果证明了AF4多检测器串联技术是一种高效的生物大分子分离表征手段。更多还原

【Abstract】 Unlike HPLC, the separation mechanism of asymmetrical flow field flow fractionation(AF4) is based on the interaction of sample and external force. AF4 has a broad determination range(1nm-100μm) due to the utilization of ‘open channel’ which requires no stationary phase or packing materials. The sample degradation is minimized in AF4. Another main advantage of AF4 is the wide choice of carrier liquid which allows for the analysis of a sample in situ. Moreover, AF4 can provide the size distribution of sample in a single run. In the present study, AF4 coupled with UV/VIS, DRI, multiangle light scattering(MALS), fluorescence(FS) was employed for the separation and characterization of germinated barley starch and egg yolk plasma. It was found that, when germinated, the apparent density and the antidiabetic activity of barley were significantly increased. The results show that AF4-UV/VIS-MALS-DRI-FS is a powder tool for the separation and characterization of biomacromolecules.更多还原