【作者】 苏东悦； 吴晓彤； 李鹿； 柴芳； 王春刚； 苏忠民；

【Author】 Su Dongyue;Wu Xiaotong;Li Lu;Chai Fang;Wang Chungang;Su Zhongmin;Department of Chemistry,Harbin Normal University;Faculty of Chemistry,Northeast Normal University;

【机构】 哈尔滨师范大学； 东北师范大学；

【摘要】 荧光铜纳米簇具有很多优于传统的荧光团的性质,如尺寸小,好的光稳定性,大的Stokes位移等,在单分子光谱,荧光成像,重金属离子检测等方面都有可观的应用前景。本实验中,我们利用叶酸作为还原剂合成叶酸功能化的荧光铜纳米簇（FA-Cu-NCs）,并且将FA-Cu-NCs作为荧光探针检测Hg²⁺。我们所合成的FA-Cu-NCs在紫外光源下,呈蓝色荧光发射。在检测过程中,当溶液中存在Hg²⁺时,荧光会在Hg²⁺与铜簇发生作用的过程被淬灭,通过对荧光的观察和荧光光谱的测试可以达到对Hg²⁺的检测目的,检测限可以达到1 nM 1,2。此外,经细胞实验证明FA-Cu-NCs具有非常好的细胞相容性,可以用作荧光成像材料。更多还原

【Abstract】 Cu NCs are considered as fluorescent probes for biolabeling and biomedical imaging due to their photophysical properties,ultrafine size and good biocompatibility,in comparison to that of organic fluorophore.In this research,folic acid functionalized gold nanoclusters（FA-Cu-NCs） were selected as a fluorescent sensor for detecting Hg²⁺.The synthesized FA-Cu-NCs solution turned on blue fluorescence emission(λ_em = 440 nm) under excited at 275 nm.The prepared FA-Cu-NCs have a long fluorescence lifetime（6.11 ns）,the absolute quantum yield of the FA-Cu-NCs was about 21%.The detection limit of mercury ion was found to be 1 nM.This will provide a fluorescence imaging materials and a simple,effective sensor for on-site and real-time Hg²⁺ detection.更多还原