【作者】 江必旺；

【Author】 Jiang,Biwang;Peking University Shenzhen Graduate School,the Center for Nano;Suzhou Nano-Micro Tech Co.,Ltd;

【机构】 北京大学深圳研究生院纳米中心； 苏州纳微科技有限公司；

【摘要】 <正>微球介质已广泛地用于多肽和蛋白的分离纯化。微球的大小,孔径分布,粒径均匀度及表面性能直接影响到生物分子的分离效果。纳微开发出最新微球合成技术可以精确控制微球的的粒径大小,孔径分布,及表面化学性能。这些微球作为色谱填料可用于高效分析和分离多肽,蛋白等生物分子。微球的性能如材料的组成,粒径大小,均匀度,孔径结构及表面功能基团是影响生物分离的最重要因素.因此根据目标分离物质的性能选择合适微球填料可以高效率,低成本地分离生物药.更多还原

【Abstract】 Significant efforts have been devoted to developing microparticle technology due to their importance in the ever expanding research, development, application and commercialization of biotechnology. Microparticles are the core materials of chromatographic packing columns which are used for analysis, separation, detection and purification of biomolecules.This presentation will focus on most recent advance in developing monodispersed microparticle technology which allows us to design and produce microparticle products of desired particle diameter. The particle size distribution is very narrow with coefficient variation CV<3%. We also develop unique coating process to completely eliminate the nonspecific binding, thus enhancing the selectivity and efficiency. It is demonstrated that highly uniform microparticles are idea chromatographic media for analysis, separation, and purification.of protein and peptide.更多还原