【作者】 唐壮； 杜伟； 李小玉； 程嘉翎；

【Author】 TANG Zhuang;DU Wei;LI Xiao-Yu;CHENG Jia-Ling;Jiangsu University of Science and Technology;Institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences;The Sericultural Research Institute, Chinese Academy of Agricultural Sciences;

【机构】 江苏科技大学； 云南省农业科学院蚕桑蜜蜂研究所； 中国农业科学院蚕业研究所；

【摘要】 转运抑制应答因子1(Transport inhibitor response 1,TIR1)作为生长素的受体,在生长素信号的感知与转导过程中发挥重要作用。从桑树品种育71-1的插穗基部的皮部中克隆到一个桑树TIR1基因,命名为MaTIR1(Gen Bank登录号:KJ787017),该基因编码区全长1 758 bp,编码585个氨基酸,蛋白质相对分子质量65 438.7,理论等电点6.31,含有F-Box结构功能域及LRRs结构功能域,不含信号肽及跨膜区。荧光实时定量PCR检测MaTIR1在桑树的果、雌花、叶、芽、茎、根中均有表达,在叶中的表达量最高,在根部的表达次之,在雌花的表达量最低,推测该基因在各组织器官的表达水平可能与各组织器官行使的生物学功能有关;在硬枝与绿枝的扦插生根过程中,MaTIR1在不定根原基形成期与不定根伸长期的表达量明显上调,提示该基因可能参与了不定根的形成。更多还原

【Abstract】 Transport inhibitor response 1(TIR1), as an auxin receptor, plays an important role in auxin signals perceiving and transmitting. In this study, we cloned a gene from mulberry cuttings and denominated Ma TIR1(Gene Bank accsssion NO. KJ787017).Sequence analysis showed that the open reading frame(ORF) of Ma TIR1 is 1758 bp, and encodes 585 amino acids. The deduced protein is predicted to have a relative molecular weight of 65438.7, and its isoelectric point was 6.31. The bioinformatics analysis results also showed that the deduced protein have the F-Box domain and LRRs domain and have neither signal peptide nor transmembrance region. Quantitative real-time PCR analysis(q RT-PCR) showed that MaTIR1 gene expressed in all tissues, and that the highest level was in leaf following root. During the mulberry hardwood cutting and greenwood cutting rooting stage, the expression level of MaTIR1 was upregulated in initiation phase and expression phases. These results suggest that MaTIR1 may involves in the formation of adventitious root.更多还原