【作者】 迟文娟； 刘文钰； 郭晓帆； 张崇； 曹远银；

【Author】 Chi Wenjuan;Liu Wenyu;Guo Xiaofan;Zhang Chong;Cao Yuanyin;Shenyang Institude of technology;College of Plant Protection,Shenyang agricultural University;

【机构】 沈阳工学院； 沈阳农业大学；

【摘要】 小麦赤霉病主要由Fusarium graminearum Schw.引起的,是一种世界各麦区发生的重要病害,能引起小麦减产和品质下降,其病原菌产生的毒素还危害人畜健康。近些年来,因缺少简便有效的检测技术影响了早期的防治处理,该病已呈现出加重趋势。为此,本文选取真菌r DNA非编码区ITS1,5.8S和ITS2的通用引物,分别对小麦赤霉病病菌等10种相关病菌进行PCR扩增、产物测序、Genbank搜索,Accelrys Gene 2.5软件比对,设计了以小麦赤霉病病菌为靶标的引物,对上述10种病菌进行检测,唯有该病菌呈阳性;设1ng-1fg 7个该病菌的DNA浓度进行灵敏度测定,表明其最低检测限为10pg DNA。该方法快速准确灵敏,为实现该病准确检测提供了关键技术支撑。更多还原

【Abstract】 wheat scab caused by Fusarium graminearum Schw. is a typical disease occurring in all countries where wheat is grown., which causing yield loss and quality reduction. Its pathogen produces mycotoxins, which affect human and animal health. In recent years, the incidence of wheat appears increasingly serious due to the lack of rapid, convenient, effective and sensitive s detection methods for the disease. Therefore, at present paper,the universal primer pair of internal transcribed spacer(ITS) located in r DNA region of fungi was chosen and used to amplify the genomic DNAs of Ustilago tritici and the rest 9 pathogens, respectively.The amplicons were sequenced, searched at Genbank and aligned using Accelrys Gene 2.5 softwere.The unique primer pair:XSHF/XSHR was designed targeted at U. tritici and used to detect all of the pathogens. It was indicated that only U. tritici appeared positive. Seven DNA concentrations from 1ng-1fg were conducted for the sensitivity test and indicated that the detection limit was 10 pg DNA, indicating that this method is rapid, accurate and sensitive and could be used for the diseased seed incidence detection.更多还原