【作者】 敖平星； 孔令青； 高洪；

【Author】 Ao Ping Xing;Kong Lingqing;Gao Hong;College of Animal Science and Technology, Yunnan Agricultural University;

【机构】 云南农业大学动物科学技术学院；

【摘要】 本研究从细胞凋亡角度,分析两种凋亡相关关键基因c-myc和Livin在肝脏细胞中的表达量变化。通过实时荧光定量RT-PCR和免疫组化试验,发现内毒素可在m RNA水平和蛋白水平诱导c-myc基因的表达并抑制Livin基因的表达,从而促进肝细胞凋亡,最终导致肝细胞损伤。我们的研究结果表明多价阳离子A作为内毒素抑制剂,能够上调肝脏Livin基因的表达和下调c-myc基因的表达,从而显著抑制肝细胞凋亡,对内毒素导致的肝细胞损伤有显著的保护效应。更多还原

【Abstract】 This study examined the changes in expression level of two apoptosis-related key genes, c-myc and Livin of hepatocytes. The results from real time PCR and immunohistochemistry showed that the endotoxin induced the expression of c-myc gene and suppressed the expression of Livin gene at the m RNA and protein levels, which further promote the hepatocyte apoptosis and eventually caused the injuries to hepatocyte. In addition,we found the multivalent cation A function as an endotoxin inhibitor, could up-regulate the expression level of Livin gene and down-regulate the expression of c-myc gene. This result suggested a protective role of multivalent cation A on hepatocytes apoptosis.更多还原