【作者】 孙雪； 段天璇； 袁瑞娟；

【Author】 SUN Xue;DUAN Tian-xuan;YUAN Rui-juan;School of Chinese Materia Medica,Beijing University of Chinese Medicine;

【机构】 北京中医药大学中药学院；

【摘要】 目的:研究蚂蟥酶解物的吸收程度,考察吸收促进剂及P-gp抑制剂对蚂蟥酶解物肠段吸收的影响。方法:采用大鼠外翻肠囊法考察蚂蟥酶解物在大鼠小肠的吸收以及加入吸收促进剂及P-gp抑制剂对蚂蟥酶解物吸收的影响。结果:不同浓度蚂蟥酶解物在大鼠小肠不同肠段的吸收较其他蛋白类药物较高。吸收促进剂组与正常组比较,蚂蟥酶解物的吸收程度无明显提高(P>0.05)。蚂蟥酶解物与维拉帕米合用后,蚂蟥酶解物在大鼠小肠中的吸收量显著增加(P<0.05)。结论:在试验浓度下,各吸收促进剂对蚂蟥酶解物的肠吸收促进作用不明显,P-gp对蚂蟥酶解物有外排作用,加入维拉帕米可增大蚂蟥酶解物的吸收。更多还原

【Abstract】 Objective:To explore the extent intestinal absorption of Whitmania pigra enzymatic extracts,and to investigate the effects of absorption enhancers and P-gp inhibitor on intestinal absorption of Whitmania pigra enzymatic extracts.Methods:A quantitative determination was established for detecting Whitmania pigra enzymatic extracts,The intestinal transport of Whitmania pigra enzymatic extracts and the effects of absorption enhancers P-gp inhibitor on intestinal absorption is performed by applying everted intestinal sacs method.Results:The ratio of extent absorption of Whitmania pigra is between 11% and 25%.Three enhancers had no significant effect on the absorption of Whitmania pigra enzymatic extracts.(P>0.05),After Whitmania pigra enzymatic extracts combined with Ver,the absorption amount of samples in rat small intestine increased significantly(P<0.05).Conclusion:Whitmania pigra enzymatic extracts has a good absorption in small intestine.The absorption enhancers cannot improve the absorption,P-gp inhibitor Ver can enhance the absorption of Whitmania pigra enzymatic extracts and increase the bioavailability.更多还原