【作者】 杨辉； 罗友华； 李成付； 黄亦琦；

【Author】 Yang Hui;Luo Youhua;Li Chenfu;Huang Yiqi;

【机构】 厦门市医药研究所药研中心厦门市天然药物研发重点实验室；

【摘要】 目的建立板蓝根药材中（R,S）-告依春高效液相含量测定方法。方法 Symmetry-C₁₈色谱柱（4.6 mm×250 mm,5μm）,流动相为甲醇-水-磷酸-三乙胺（14.0:85.3:0.6:0.1）,流速1.0 mL·min^-1,柱温为室温,检测波长245 nm。结果 （R,S）-告依春在0.0025⁰.2480μg范围内与峰面积成良好线性关系（r=0.9999）,回收率平均为98.9%,RSD为1.9%。结论本方法快速、准确,精密度、稳定性、重复性良好,可以测定板蓝根药材中（R,S）-告依春的含量。更多还原

【Abstract】 OBJECTIVE To establish a method for the determination of（R,S）-epigoitrin of Isatis indigotica Fort.by HPLC.METHODS The chromatographic conditions were as follows:Symmetry-C₁₈ analytic chromatographic column（4.6mm × 250 mm,5 μm）;The mobile phase consisted of methanol-water-phosphoric acid-triethylamine（14.0:85.3:0.6:0.1）,eluted at a rate of 1.0 mL·min^-1;column temperature:room temperature.The wavelength for detection was245 nm.RESULTS The standard curves of（R,S）-epigoitrin was linear in the concentration range of 0.0025-0.2480 μg,r = 0.9999;the average recovery was 98.9%,with RSD of 1.9%.Conclusion The precision,stability and repeatability were fme.This method is sensitive and quick for the determination of（R,S）-epigoitrin in Isatis indigotica Fort..更多还原