【作者】 雷有成； 李建蕊； 肖佳佳； 张兴国； 张梅； 吕光华；

【Author】 LEI You-cheng;LI Jian-rui;XIAO Jia-jia;ZHANG Xin-guo;ZHANG Mei;LU Guang-hua;Key Laboratory of the Ministry of Education in China on the Standardization of Chinese Materia Medica,School of Pharmacy,Chengdu University of Traditional Chinese Medicine;Southwest Jiaotong University;

【机构】 成都中医药大学药学院,中药资源系统研究与开发利用国家重点实验室培育基地,中药材标准化教育部重点实验室； 西南交通大学中药研究所；

【摘要】 目的研究天麻中活性成分天麻多糖含量与天麻商品规格、等级和产地之间的相关性,评价其质量。方法建立苯酚-硫酸法测定天麻多糖含量的方法;收集、测定了34份冬天麻、春天麻及其不同等级和产地的样品,结合性状鉴别特征,分析天麻多糖含量与这些因素之间的关系。结果冬天麻中天麻多糖的含量(26.05%,n=24)高于春天麻(20.21%,n=10);在不同等级的天麻中,天麻多糖含量为一等(32.65%,n=6)、二等天麻(32.89%,n=6)高于三等(21.71%,n=8)、四等天麻(14.57%,n=4)。四川、陕西、云南等天麻传统产地的天麻样品中多糖含量较其他产地高。结论建立的苯酚-硫酸法可用于准确测定天麻中多糖的含量。天麻多糖的含量与天麻商品规格、等级和经验性状鉴别优劣的结果相符合,可作为天麻质量评价的指标。更多还原

【Abstract】 Objective To study the correlation of active ingredients content of polysaccharides,and the commercial specifications,grades and origins of Gastrodia tuber,then evaluate herbal quality.Methods Content of polysaccharides was measured by phenol- sulfuric acid method.34 Winter,Spring Gastrodia tuber samples with different grades and origins were collected and measured.Combined with the morphological identification,the correlation of these factors and active ingredient content were analyzed.Results The content of polysaccharides in Winter Gastrodia tuber(26.05%,n = 24) is higher than Spring Gastrodia tuber(20.21%,n = 10).That polysaccharides in the first grade(32.65%,n = 6) and second grade(32.89%,n = 6) is higher than third grade(21.71%,n = 8) and fourth grade(14.57%,n = 4).As to the content of polysaccharides,the content samples of Sichuan,Shanxi and Yunnan province are better than others.Conclusion The method is accurate and reliable for determination of polysaccharides in Gastrodia tuber.The content of polysaccharides is consistent with commercial specifications,grades and traditional experience for quality evaluation of Gastrodia tuber.Moreover it can be used as the evaluation index of Gastrodia tuber quality.更多还原