【作者】 郑媛； 张建军； 王淳； 王林元； 杨旭； 刘畅；

【Author】 ZHENG Yuan;ZHANG Jian-jun;WANG Chun;WANG Lin-yuan;YANG Xu;LIU Chang;Beijing University of Chinese Medicine;

【机构】 北京中医药大学；

【摘要】 目的:研究以消石利尿化瘀法创制的中药复方对高尿酸血症大鼠血清UA、NO、ET-1、TXB₂及6-keto-PGF1a含量的影响及其对血管内皮功能的保护作用。方法:将雄性SD大鼠随机分为空白组、模型组、苯溴马隆组及中药复方高、中、低剂量组,共6组。除空白组外,其余各组均以腺嘌呤和酵母膏灌胃,同时各给药组灌胃给予相应的药物,空白组和模型组给予等体积蒸馏水,连续21天。末次给药后,采用紫外分光光度法检测大鼠血清UA及NO的含量,放射免疫分析法检测ET-1、TXB₂及6-keto-PGF_1a的含量。结果:（1）模型组大鼠血清UA、ET-1、TXB₂含量与空白组比较,显著升高（P<0.05）;NO、6-keto-PGF_1a含量与空白组比较,显著降低（P<0.05）。（2）中药复方中剂量组大鼠血清UA、ET-1、TXB₂含量较模型组显著降低（P<0.01）。（3）中药复方中剂量组大鼠血清NO、6-keto-PGF_1a含量较模型组显著升高（P<0.01）。结论:以消石利尿化瘀法创制的中药复方在降低高尿酸血症大鼠血清尿酸的同时,对实验动物血管内皮的舒缩功能有保护作用。更多还原

【Abstract】 Objective:To investigate the uric acid（UA）,nitric oxide（NO）,Endothe lin-1（ET-1）,Thromboxane B₂（TXB₂） and 6-keto-prostaglandin F1a（6-keto-PGF1a） in the rats with Hyperuricemia of Chinese herbal compounds prescription,which based on the traditional therapeutics of expelling stone,diuresis and removing stasis and investigate the mechanism and its protection on function of vascular endothelial cells（VEC）.Methods:Male SD rats were randomly divided into normal group,model group,Benzbromarone group,high medium and low-dose Chinese herbal compounds Prescription（HC,MC and LC） group in three doses.In addition to the normal group,the rest ones were offered with Adenine and Yeast extracts for 21 days.While the appropriate drugs administered to the rats of the administration group,the normal group and the model group were given by gavage the distilled water for 21 days.Then blood was collected,the UA and NO content in serum were determined by ultraviolet-visible spectroscopy,the ET-1,TXB₂and 6-keto-PGF1 a content in serum were detected by radiommunoassay.Results:The level of UA,ET-1,TXB2 in the model group Compared to the normal group was significantly increased（P<0.05）.The level of NO,6-keto-PGF1 a in the model group Compared to the normal group was significantly decreased（P<0.05）.Compared with the model group,the concentration of UA,ET-1and TXB2 evidently decreased in serumin MC group of treated rats（P<0.01）.Compared with the model group,the concentration of NO and 6-keto-PGF1 a in each Chinese herbal compounds prescription group was evidently increased in serumin（P<0.01）.Conclusions:The Chinese herbal compounds prescription which based on the traditional therapeutics of expelling stone,diuresis and remove stasis decreased the uric acid content and protected the function of vasomotor.更多还原