【作者】 王君明； 荣春蕾； 李晓冰； 张月月； 陈玉龙； 陈辉； 崔瑛； 屈凌波；

【Author】 WANG Jun-ming;RONG Chun-lei;LI Xiao-bing;ZHANG Yue-yue;CHEN Yu-long;CHEN Hui;CUI Ying;QU Ling-bo;Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province,Henan University of Traditional Chinese Medicine;College of Chemistry and Molecular Engineering,Zhengzhou University;School of Chemistry and Chemical Engineering,Henan University of Technology;

【机构】 河南中医学院/呼吸疾病诊疗与新药研发河南省协同创新中心； 郑州大学化学与分子工程学院； 河南工业大学化学化工学院；

【摘要】 目的:首次考察雷公藤（LGT）配伍金钱草（JQC）对人食管癌Eca9706细胞抗癌增效作用的组成配比及其量效关系。方法:将LGT与JOG按照不同质量配比（1/4、1/2、1/1、2/1、4/1）混合后,分别用水和75%乙醇回流提取,制备LGT-JQC不同质量配比的提取物:采用体外MTT法检测LGT-JQC不同配比配伍提取物（200μg/mL）对人Eca9706细胞增殖抑制的影响,确定LGT-JQC配伍增效作用的最佳组成配比;进一步采用体外MTT法检测最佳组成配比的提取物对人Eca9706细胞增殖抑制作用的量效关系,并通过SPSS统计软件计算其半数抑制浓度(IC₅₀)值。结果:LGT-JQC在质量配比2/1⁴/1配伍的醇提物（200μg/mL）具有抑制人Eca9706细胞增殖的增效作用（P<0.05）,且配比在2/1时增效作用最强（与配比4/1比较,P<0.05）,而LGT-JQC配伍的水提物（200μg/mL）在既定配比下均无配伍增效作用（P>0.05）;LGT-JQC配比在2/1（最佳配比）的醇提物,在浓度为50⁴00μg/mL范围内对Eca9706细胞的增殖抑制呈现出良好的剂量依赖性关系,其IC₅₀值为316.28μg/mL。结论:LGT-JQC配伍的醇提物对体外人食管癌Eca9706细胞增殖的抑制具有协同增效作用,二者组成配比应在2/1⁴/1,且尤以在2/1作用为佳;LGT-JQC配比为2/1的醇提物,在浓度为50⁴00μg/mL对人Eca9706细胞的增殖抑制具有良好的量效关系。更多还原

【Abstract】 Objective:To investigate the component ratio and dose-effect relationship of Tripterygium wilfordii（LGT） combined with Lysimachia Christina（JQC） in inhibiting Eca9706 esophageal cancer cells in vitro,for the first time.Methods:Reflux extraction was used to prepare water or 75% ethanol extracts in different mass component ratios（1/4,1/2,1/1,2/1,4/1） of LGT and JQC.MTT assay was used to test the inhibition of cell proliferation and thus to determine the component ratio and dose-effect relationship of LGT combined with JQC in inhibiting Eca9706 cells in vitro.Besides,SPSS statistical software was used to calculate the 50% inhibitory concentration(IC₅₀).Results:Ethanol extracts（200 μg/mL） of LGT-JQC in ratio of 2/1 to 4/1 had synergistic effect in inhibiting Eca9706 cell proliferation（P <0.05）,and the effect of LGT-JQC in the 2/1 ratio was stronger than that of the 4/1 one（P <0.05）,while the water extracts（200 μg/mL）of LGT-JQC in each given component ration had no synergistic effect（P > 0.05）.The ethanol extract of LGT-JQC in the 2/1 ratio at a concentration of 50-400 μg/mL range all inhibited the proliferation of Eca9706 cells and showed a good dose-dependent manner,and its IC50 value was 316.28 μg/mL.Conclusion:The ethanol extract of LGT-JQC compatibility had synergistic effect on Eca9706 cell proliferation in vitro,the mass component ratio should be at 2/1 to 4/1,and in particular the role better in 2/1.The ethanol extract of LGT-JQC in 2/1 ratio at a concentration of 50⁴00 μg/mL inhibited the proliferation of Eca9706 cells with good dose-effect relationship.更多还原