【作者】 付晓伶； 任建琳； 隋华； 潘树芳； 周利红； 李琦；

【Author】 SUI Hua;PAN Shu-fang;JIN Bao-hui;ZHU Hui-rong;LI Qi;Department of Clinic Oncology,Putuo Hospital and Interventional Cancer Institute of Chinese Integrative Medicine,Shanghai University of Traditional Chinese Medicine;Deparment of Medical Oncology,Shuguang Hospital,Shanghai University of Traditional Chinese Medicine;

【机构】 上海中医药大学附属曙光医院肿瘤科； 上海市中医医院肿瘤科；

【摘要】 目的:探讨左金丸（ZJW）对人结肠癌多药耐药细胞HCT-116/L-OHP的逆转作用及其可能机制。方法:人结肠癌HCT-116细胞和HCT-116/L-OHP多药耐药细胞常规培养,左金丸干预48h后,MTT法检测HCT-116/L-OHP细胞对化疗药敏感性的影响,电感耦合等离子质谱法（ICP-MS）检测耐药细胞内奥沙利铂（L-OHP）药物浓度的改变,流式细胞仪分析细胞凋亡情况。结果:左金丸增加HCT-116/L-OHP细胞对L-OHP、顺铂（DDP）、五氟尿嘧啶（5-Fu）、丝裂霉素C（MM）C四种化疗药物的敏感性,四种化疗药的IC₅₀分别从（157.48±16.73）μg/mL下降到（71.40±6.48）μg/mL;（26.38±1.92）μg/mL下降到（16.12±1.60）μg/mL,（33.29±3.64）μg/mL下降到（13.11±2.65）μg/mL,（12.50±2.49）μg/mL下降到2.71±1.10μg/mL;左金丸干预HCT-116/L-OHP细胞48h后细胞内L-OHP浓度明显增高（P<0.01）,耐药细胞凋亡率显著增加（P<0.01）。结论:左金丸通过增加结肠癌多药耐药细胞内化疗药物浓度,抑制细胞增殖,诱导其凋亡,逆转HCT-116/L-OHP细胞株的多药耐药性。更多还原

【Abstract】 Objective:To investigate the mechanisms and reversal effects of Zuo Jin Wan(ZIW) on human colon carcinoma multidrug resistance(MDR) in HCT-116/L-OHP cells.Methods:Human colon carcinoma MDR cells were cultured conventionally.The concentration of ZJW combined with chemotherapy drugs and the reversal effect of multidrug resistance(MDR) in HCT-116/L-OHP cells was evaluated by the MTT assay.The variation of intracellular concentration of L-OHP in HCT-116/L-OHP cells was evaluated by the ICP-MS,and the effects of ZJW on L-OHP induced apoptosis and cell cycle were evaluated by flow cytometry(FCM).Results:ZJW could increase the sensitivity of HCT-116/L-OHP cells to L-OHP,DDPs5-Fu,MMC.The IC50 value of L-OHP decreased from(157.48±16.73)μg/mL to(71.40±6.48)μg/mL,DDP was from(26.38±1.92)μg/mL to(16.12±1.60)μg/mL,5-Fu was from(33.29±3.64)μg/mL to(13.11±2.65) μg/mL,and MMC was from(12.50±2.49)μg/mL to(2.71±1.10) μg/mL.ICP-MS results showed that ZJW increased the concentration of drugs in HCT-116/L-OHP cells after treatment for 48 h.Flow cytometry assay result verified that the increased effects of ZJW combined with L-OHP on the apoptosis of HCT-116/L-OHP cells.Conclusion:ZJW reverse MDR in HCT-116/L-OHP cell,which possibly through inhibiting cell proliferation,inducing cell-apoptosis,ultimately affect the concentration of drugs inside the cells.更多还原