【作者】 蔡玲玲； 张丰川； 李元文； 张小静； 孙毅坤； 李海聪；

【Author】 CAI Ling-ling;ZHANG Feng-chuan;LI Yuan-wen;Dermatological Department,Dongfang Hospital of Beijing University of Chinese Medicine;Pharmacy college of Beijing University of Chinese Medicine;

【机构】 北京中医药大学东方医院皮肤科； 北京中医药大学药学院；

【摘要】 目的观察苦参碱对合轴马拉色菌麦角固醇生物合成的影响,探讨苦参碱抑菌机理。方法方法通过高效液相色谱法(HPLC)检测苦参碱、酮康唑两组药物作用后合轴马拉色菌麦角固醇含量。结果苦参碱组与酮康唑组均有抑制麦角固醇合成的作用无统计学差异(P=0.65>0.05),说明两组作用相当。两组药物对于合轴马拉色菌临床株和标准株的抑制强弱有明显差异(P=0.02<0.05),两组药物均对于标准菌株抑菌效果明显强于临床菌株。结论苦参碱可以抑制合轴马拉色菌麦角固醇合成,作用与酮康唑相当,考虑其抑菌机制可能与破坏麦角固醇合成有关。更多还原

【Abstract】 Objective We tested M.sympodialis ergosterol concentrations by HPLC to discuss mechanism on matrine inhibition of malassezia.Methods We checked ergosterol concentrations of M.sympodialis after matrine or ketoconazole affected by High Performance Liquid Chromatography(HPLC).Results Two groups both can inhibite ergosterol(P=0.65>0.05),and have no significance.Two groups had abvious differences of malassezia inhibitions on clinical strains when its compared with standard strains,two treatment groups both had stronger inhibition in standard groups than clinical groups(P=0.02 < 0.05).Conclusion 1.Matrine has inhibitions of ergosterol of M.sympodialis as well as ketoconazole.Its inhibition mechanism of M:sympodialis might have relationship of inhibition of ergosterol biosynthesis.更多还原