【作者】 张彩晴； 曹伟； ZHENG Long； WANG Qian-yun； MA xiao-ying； LI Shi-mei；

【Author】 ZHANG Cai-qing;ZHENG Long;WANG Qian-yun;MA xiao-ying;LI Shi-mei;

【机构】 解放军第323医院； 宝鸡市中心医院；

【摘要】 目的研究阿维A对HaCaT细胞核转录因子-κappaB（Nuclear factor-kappa B,NF-κB）表达的影响,探讨阿维A治疗皮肤病的非受体作用机制。方法细胞共分5组:空白对照组、对照组(10^-5mol/L P物质),小剂量组(10^-8mol/L阿维A+10^-5mol/L P物质),中剂量组(10^-7mol/L阿维A+10^-5mol/L P物质)、大剂量组(10^-6mol/L阿维A+10^-5mol/L P物质)。通过不同浓度的阿维A对HaCaT细胞进行干预,应用免疫细胞化学法、western-blot、RT-PCR法检测HaCaT细胞中NF-κB阳性细胞表达率、细胞蛋白和基因表达的变化。结果与空白对照组相比,对照组阳性细胞表达率、细胞蛋白、基因的表达均增强。与对照组相比,各阿维A组阳性细胞表达率、蛋白和mRNA表达均降低（P<0.05或P<0.01）。结论阿维A通过对人角质形成细胞细胞核转录因子-κB的调控是其治疗皮肤病的作用途径之一。更多还原

【Abstract】 Objective To investigate the effects of acitretin on the expression in nuclear factor-kappa B of HaCaT cells.To explore the non-receptor mechanism of retinoic acid in treating skin diseases.Methods The cells were classified into blank group,control group(10^-5mol/L SP),low dose group(10^-8mol/L acitretin +10^-5mol/L SP),moderate dose group(10^-7mol/L acitretin +10^-5mol/L substance P) and larger dose group(10^-6mol/L acitretin +10^-5mol/L Substance P).Then,expressions of NF-κB in positive cells,cell protein and mRNA were detected by the means of immunocytochemical technique,Western-blot method and RT-PCR method,respectively.Results Compared with the blank group,the expression rate of positive cells,the relative expression value of NF-κB mRNA and cell protein increased,and had a significant difference（P<0.05）.Compared with the control group,all of them in the acitretin groups decreased significantly（P<0.05 or P<0.01）.Conclusion To regulate the expression of nuclear factor-kappa B in HaCaT cells is a way of acitretin in treating skin diseases.更多还原