【作者】 章常华； 涂秀英； 魏学鑫； 李瑛； 徐国良；

【Author】 ZHANG Chang-hua;TU Xiu-ying;WEI Xue-xin;LI Ying;XU Guo-liang;Department of Pharmacology,College of Pharmacy,Jiangxi University of Traditional Chinese Medicine(TCM);Research Center for Differentiation and Development of Basic theory of TCM,Jiangxi University of TCM;

【机构】 江西中医学院药学院； 江西中医药大学中医基础理论分化发展研究中心；

【摘要】 目的:探讨小檗碱和黄芩苷对葡萄糖氧化酶(GOD)法测定葡萄糖含量的干扰作用。方法:用GOD法测定加入不同浓度小檗碱和黄芩苷的含血清培养基葡萄糖含量结果,对其结果进行分析、比较。结果:对照组与小檗碱和黄芩苷给药组间葡萄糖含量无显著差异,表明小檗碱和黄芩苷对GOD测定葡萄糖含量无明显干扰作用(1-200μmol/L),结论:在用GOD法测定葡萄糖含量时,小檗碱和黄芩苷对GOD测定葡萄糖含量无明显干扰作用(1-200μmol/L)。更多还原

【Abstract】 Objective:To investigate the interference effect of berberine and baicalin on glucose oxidase assay in determination of the content of glucose.Methods:To determine glucose concentration of medium containing different concentrations of berberine and baicalinby the method of glucose oxidase.Results:There was no significant change between the berberine group and the control(P>0.05),indicating that berberine and baicalin themselves at the concentration(1-200 umol/L) used in the experiment do not cause interference.Conclusion:The experimental results indicated that berberine and baicalin themselves at the concentration(1-200 umol/L) used in the experiment do not cause interference.更多还原