【作者】 胡鹏翼； 吴清； 郑琴； 张国松； 龚莹莹； 王佳； 杨明；

【Author】 HU Peng-yi;Wu Qing;ZHENG Qin;ZHANG Guo-song;GONG Ying-ying;WANG Jia;YANG Ming;Key Laboratory of Modern Preparation of Jiangxi University of Traditional Chinese Medicine,Ministry of Education;School of Chinese Materia Medica,Beijing University of Chinese Medicine;

【机构】 江西中医药大学现代中药制剂教育部重点实验室； 北京中医药大学中药学院；

【摘要】 目的确定槐定碱热敏脂质体最佳处方及制备工艺。方法以热敏脂质体分别于37℃、43℃累积释药百分率为评价指标,采用design-expert软件进行中心复合设计,建立各指标与因素之间的数学关系,通过效应面法优化处方工艺条件,通过验证性实验进行预测分析。结果中心优化设计考察得最佳处方工艺条件:脂药比为17.80,DPPC/MSPC为9.50制得的脂质体在37℃时释药达到4.30%,43℃释药79.03%,包封率为(81.33±1.24)%。结论以pH梯度结合逆向法制备槐定碱热敏脂质体包封率较高,热敏释药性质良好。更多还原

【Abstract】 Objective To optimize the formulation and preparation parameters of sophoridine thermosentitive liposomes.Method The cumulative release percents of sophoridine thermosensitive liposomes at 37℃ and 43℃ respectively were used as the influence factors,Design-expert was used to establish the mathematical relationship of parameters and factors,and response surface method was used to optimize formulation and process.The experimental results of the thermosensitive liposomes prepared under the optimum condition were compared with the predicted value.Result s Optimal conditions were that the ratio of sophoridine and DPPC was17.80,the ratio of DPPC and MSPC was 9.50.In such conditions,the cumulative release percents of sophoridine thermosensitive liposomes at 37℃ and 43℃ were 79.03%and 79.03%respectively,and the entrapment efficiency was(81.33±1.24%).Conclusion The sophoridine thermosensitive liposomes with high entrapping efficiency and good thermal drug release properties can be prepared by pH-gradient method coupled with reverse evaporation technique.更多还原