【作者】 康林之； 任刚； 罗云； 张婧； 张丽； 刘荣华； 杨明； 赵海平；

【Author】 Kang Lin-zhi;Ren Gang;Luo Yun;Zhang Jing;Zhang Li;LIU Rong-hua;Yang Ming;Zhao Hai-ping;College of Nursing,Jiangxi University of Traditional Chinese Medicine;Key Laboratory of Modern Preparation of TCM,Ministry of Education,Jiangxi University of Traditional Chinese Medicine;College of Pharmacy,Jiangxi University of Traditional Chinese Medicine;

【机构】 江西中医学院护理学院； 江西中医学院现代中药制剂教育部重点实验室； 江西中医学院药学院；

【摘要】 目的:研究红管药抗慢支组分平喘的最佳配比及急性毒性。方法①合格豚鼠72只,300±20g,雄性,随机分为空白组、氨茶碱组(0.047g·kg^-1)、红管药总皂苷与总黄酮不同配比组(8:1、4:1、2:1、1:1、1:2、1:4、1:8,0.38 g·kg^-1)。各给药组连续灌胃给药3d,1次·d^-1,采用His-Ach雾化引喘法通过诱咳引喘仪优选最佳配比;②制备豚鼠离体气管环标本,采用Powerlab生物信号采集系统,考察在正常克氏液条件下加入Ach后豚鼠平滑肌条的收缩状况,以及其他给药组分别于Ach致平滑肌收缩曲线上升到最明显时,加入氨茶碱(1.25mg·ml^-1),各配比药液(0.02g·ml^-1)的舒张百分率。③采用经典的小鼠急性毒性试验方法,考察红管药总皂苷与总黄酮的急性毒性。结果总皂苷和总黄酮各配比中1:2、1:4组的引喘潜伏期与氨茶碱组未有显著性差异（P>0.05）;各配比组对Ach致痉豚鼠气管平滑肌的舒张百分率不及氨茶碱组（P<0.05或P<0.01）,但其中以1:1、1:2、1:4组与之最接近。红管药总皂苷LD₅₀约为24.03g/kg,红管药总黄酮LD₅₀为20.21g/kg。结论在给药总量恒定的情况下,红管药总皂苷与总黄酮1:2,1:4平喘解痉作用明显,可能两者存在协同增效的配伍关系。红管药总黄酮和总皂苷的LD₅₀属于无毒级。更多还原

【Abstract】 Objective:To screen the optimal compositions of Aster ageratoides Turcz.for relieving asthma and to study their acute toxicity.Methods:①Seventy-two qualified male guinea pigs,300±20g,were randomly divided into the normal group,aminophylline group（0.047g/kg） and groups of different proportions of total saponins and total flavonoids（8:1,4:1,2:1,1:1,1:2,1:4,1:8）.Every group was ig administrated at dose of 0.38g/kg once a day for 3 days.The effect of relieving asthma was taken as the indicators for the optimization of compositions,which was detected by observing the latent period of asthma induced by acetylcholine-histamine in guinea pigs.②The relaxation percentage of tracheal ring’s smooth muscle retracted by acetylcholine was detected by Powerlab biological signal collecting system when aminophylline（1.25mg/ml） and different matching drugs（0.02g/ml） were added in Rrebs’ solution.③The acute toxicity of total saponins and total flavonoids was detected by classical acute toxicity test of mouse.Results:①The latent periods of asthma of group l:2（total saponins:total flavonoids） and group 1:4 had no significant differences with the aminophylline group（P>0.05）.②The relaxation percentage of tracheal ring’s smooth muscle retracted by acetylcholine of all different matching groups were not more than the aminophylline group（P<0.05 or P<0.01）,but group 1:1,group 1:2 and group 1:4 were get close to the aminophylline group.③LD₅₀ of total saponins and total flavonoids were about 24.03 g/kg and 20.21g/kg,respectively.Conclusions:For the same total dosage,the relieving asthma effects of proportions of 1:2 and l:4（total saponins and total flavonoids） of Aster ageratoides Turcz were obvious.Synergetic compatibility relationship between total saponins and total flavonoids maybe exist.The total saponins and total flavonoids of Aster ageratoides Turcz had no acute toxicity.更多还原