【作者】 秦红玉； 许静； 李能琴； 周建波； 黄怡； 朱亚静； 朱敏； 王士长；

【Author】 QIN Hong-yu;XU Jing;LI Neng-qin;ZHOU Jian-bo;HUANG Yi;ZHU Ya-jing;ZHU Min;WANG Shi-chang;Animal science and technology of Guangxi University;

【机构】 广西大学动物科学技术学院；

【摘要】 从采食富含单宁的植物的山羊瘤胃中,通过单宁浓度耐受培养基和单宁酶活力鉴定培养基分离、筛选到4株降解单宁的细菌,编号为NO.1、NO.2、NO.3、NO.4,并通过16S rRNA基因序列测定分析对4株菌进行了初步鉴定,分别为NO.1产碱普罗威登斯菌(Providencia alcalifaciens),NO.2 Kerstersia,NO.3 Providencia Vermicola,NO.4铜绿假单胞菌(Pseudomonas aeruginosa)。将菌株在单宁含量为0.5%、1.0%、1.5%的液体培养基中诱导,进行了胞内酶活测定,其中菌株Kerstersia的单宁酶活力明显高于其他三株菌,而其他三株菌的酶活并无太大差异。经1%单宁浓度组诱导的四株菌的单宁酶活,普遍高于0.5%和1.5%单宁浓度组。更多还原

【Abstract】 Strains were isolated from rumen of goat feed on plants rich in tannin,which can degrade tannin,through the isolation and identification of the tannin concentration tolerated medium and tannase activity medium,we get four highly enzymatic active.strains No.1,No.2,No.3,and No.4,respectively called Providencia alcalifaciens,Kerstersia,Providencia Vermicola,and Pseudomonas aeruginosa.The strain induced in the tannin content is 0.5%,1.0%,1.5%of the liquid medium,and carried out intracellular enzyme assay the tannase activity of Kerstersia is higher than the others,but there is not much difference among the others.The tannase activities of 4 bacterial strains induced through the group with concentration of 1%Tannin are generally higher than the groups with concentrations of 0.5%and 1.5%Tannin.更多还原