【作者】 姚付军； 李向军； 袁倬斌；

【Author】 Fujun Yao;Xiangjun li;Zhuobin Yuan;Graduate School of the Chinese Academy of Sciences;

【机构】 中国科学院研究生院；

【摘要】 <正>阿尔茨海默病是一种神经退行性疾病,其主要病理特征为大脑皮质、海马中存在淀粉样β蛋白沉积1。研究表明过渡金属离子,例如,Zn2+和Cu2+与淀粉样β蛋白的相互作用是影响沉积生成的主要原因2-5。本文采用表面等离子共振法分别研究了人体中淀粉样β蛋白(h Aβ)和小鼠体中淀粉样β蛋白(r Aβ)与Zn2+、Cu2+两种金属离子的相互作用。研究表明h Aβ与Zn2+的结合方式同离子浓度存更多还原

【Abstract】 Amyloid β peptide plays a critical role in the pathogenesis of Alzheimer’s disease(AD). Transition metals, such as Cu2+ and Zn2+ are found in Aβ plaques, and they have been shown to affect Aβ aggregation. Here we investigated the interactions between metal ions and Aβ1-16 by a flow-injection surface plasmon resonance spectrometry(SPR). Our study revealed that both Zn2+ and Cu2+ promoted human Aβ1-16 conformation change but the Zn-induced confromational change is more stable. However, rat Aβ1-16 binds Zn2+ less avidly and hardly no conformational change, this may due to three point subsitutions, R5 G, Y10 F, and H13 R in rat Aβ1-16 which appear to alter the physicochemical properties of the peptide. The results demonstrate that SPR biosensor can be a sensitive and effective method to investigate the interaction mechanism between metal ions and amyloid β-peptide.更多还原