【作者】 符莹； 张新爱； 滕瑛巧； 许丽莉； 张文； 金利通；

【Author】 Ying Fu;Xinai Zhang;Yingqiao Teng;Lili Xu;Wen Zhang;Litong Jin;Department of Chemistry, East China Normal University;

【机构】 华东师范大学化学系；

【摘要】 <正>多聚糖(glycans)作为细胞中普遍存在的糖基化产物,在很多生物学过程中起着关键作用,如细胞黏附,分子运输和清除,受体激活,以及信号转导等,更为重要的是多聚糖表达水平上的差异与肿瘤细胞的侵袭和转移有密切关系[1]。因此,准确、灵敏地测定多聚糖对于肿瘤疾病的早期诊断从而提高病人的生存几率具有极其重要的意义[2~3]。近年来,生物传感器技术由于具有可实现在体检测、更多还原

【Abstract】 Cell surface glycans are involved in cell-cell interaction and communication, cell adhesion, and many developmental processes. More importantly, the abnormal alteration of glycan expression on cell surface is associated with plenty of diseases, especially in cancers. Therefore, sensitive detection of carbohydrates on living cells is of urgent need for basic science advancement and clinical diagnostics. In this paper, we report a novel lectin-based biosensor for competitive assay of glycan espression on living cells. The biosensor was prepared by using Nafion/Au nanorod(GNR) layer to capture concanavalin A(Con A). {QDs-BSA-Au-S-mannose} bioconjugate was also synthesized by employing the BSA-protected Au nanoparticles(Au NPs) as matrixes to load Quantum Dots(QDs) for fluorescence signal generation. Thiomannosyl dimer was further incubated for competitive binding electrode-modified Con A with cell surface mannose. This proposed biosensor provides a reliable approach for in situ evaluation of the glycan expression on living cells and may facilitate the medical diagnosis and treatment.更多还原