【作者】 朱玲艳； 王宗花； 郭新美； 张菲菲； 夏建飞；

【Author】 ZHU Ling-yan;WANG Zong-hua;GUO Xin-mei;ZHANG Fei-fei;XIA Jian-fei;Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, College of Chemical and Environment Engineering, Qingdao University;

【机构】 青岛大学纤维新材料与现代纺织实验室 国家重点实验室培育基地 化学化工与环境学院；

【摘要】 <正>芦丁和槲皮素在药物中经常会由于某种原因同时存在,由于组成成分在中药医学中是非常重要的,所以发展一种方法用于天然产物或天然药物中芦丁和槲皮素成分的鉴定是非常必要的。因为大多数的黄酮类物质在适合的氧化电位下,都是具有电化学活性的。电化学的方法具有更高的灵敏度和较低的来自于无电化学更多还原

【Abstract】 A novel voltammetry method has been developed for simultaneous determination of rutin and quercetin. A novel graphene modified electrode(GH/GC) for this purpose was constructed by dropping 3 μL dispersion of graphene at the surface of glassy carbon electrode. GH was prepared as the modified Hummers method mentioned in the literature. Duo to the π-π conjugation between graphene and rutin, strong adsorption and favorable assembly process of this specie were observed on GH/GC surface, resulting in considerably increased signals with well separated voltammetric peaks of rutin and quercetin, allowing simultaneous detection of these species(Fig. 1). Take the rutin for example, the electrochemical behaviors of rutin was investigated using cyclic voltammetry(CV) and differential pulse voltammetry(DPV) at GH/GC. The electrochemical system was optimized for the selection of a suitable buffer system and scan rate. Finally, the GH/GC, as an electrochemical sensor, gave detecting sensitivities as high as 9.0 ×10-9 mol/L(quercetin) in the linear range of 1.5×10-8-5.0×10-3 mol/L and 1.2×10-8 mol/L(rutin) in the linear range of 3.0×10-8- 1.2×10-3 mol/L. The applicability of the method to real sample analysis was also evaluated.更多还原