【作者】 吴萍； 蔡称心；

【Author】 Ping Wu;Chenxin Cai;Jiangsu Key Laboratory of New Power Batteries, College of Chemistry and Materials Science, Nanjing Normal University;

【机构】 南京师范大学化学与材料科学学院,江苏省新型动力电池重点实验室,江苏省生物功能材料重点实验室；

【摘要】 <正>细胞在代谢过程中不断产生各种活性氧(ROS),它们作为信号分子或第二信使参与多种因子细胞生物学效应的启动,但同时也可与细胞内的生物大分子反应,从而直接引起细胞膜脂质过氧化、细胞内蛋白质和酶类变性、核酸DNA损伤等,最终导致细胞死亡、组织损伤、心血管疾病、肿瘤及神经元退变等多种疾病的发生[1,2]。过氧化氢是ROS中最为稳定的一种,最新的研究发现过氧化氢更多还原

【Abstract】 Developed a new method for reliable determination of cellular ROS(reactive oxygen species) concentration could lead to a better understanding of the clinical consequences of the enhancement in ROS concentration, elucidating the relationship between ROS and environmental stresses and lipid peroxidation, and assisting in studies of the biological effect of ROS in cells. This work developed and validated an electrochemical approach for measuring the flux of H2O2(a major ROS in living organisms) releasing from RAW 264.7 macrophage cells. This approach is based on the electrocatalytic reduction of the releasing H2O2 at the biosensor of HRP-attapulgite/GC, which was fabricated by depositing the horseradish peroxidase-attapulgite nanohybrids on the glassy carbon(GC) electrode. The biosensor exhibited a rapid response(less than 2 s), a wide linear range(0.2 to 150 μM), a high sensitivity(55.8 ± 2.9 μA m M–1 cm–2), a low detection limit(0.05 ± 0.01 μM), as well as good stability and repeatability due to using the natural mineral(attapulgite) as the enzyme immobilization substrate. Therefore, this work has demonstrated a simple and effective sensing platform for detection of cellular H2O2 released from cells such as RAW 264.7 cells, which has potential utility to cellular biology and pathophysiology.更多还原