【作者】 丁彩凤； 王振峰； 钟华； 张书圣；

【Author】 Caifeng Ding;Zhenfeng Wang;Hua Zhong;Shusheng Zhang;State Key Laboratory Base of Eco-chemical Engineering,College of Chemistry and Molecular Engineering,Qingdao University of Science and Technology;

【机构】 青岛科技大学化学与分子工程学院,生态化工国家重点实验室培育基地；

【摘要】 <正>本文通过氨基与羧基的脱水缩合作用将适体修饰于磁性微球表面,将探针DNA及前体DNA修饰到聚苯乙烯微球表面制备纳米探针。然后通过适体DNA与探针DNA的部分杂交,形成纳米生物复合体。环状模板DNA与前体DNA部分杂交后,在DNA连接酶、DNTPs及Φ29DNA聚合酶作用下,开始滚环复制过程,形更多还原

【Abstract】 Aptame based rolling circle amplification(aptamer-RCA) was developed as a novel versatile electrochemical platform for ultrasensitive detection of cancer cells. The primer sequence on the nanoprobes, which were displaced by the target cells, mediated a RCA reaction that generated hundreds of copies of a circular DNA template. Detection of the Cd S nanoparticles modified on the signal DNA which was complementary to the copies of the circular DNA template allowed enormous sensitivity enhancement in the assay of target cells. Because of the wide availability of aptamers for numerous cancer cells, this method holds great promise in clinical application.更多还原