【作者】 洪敏； 朱进； 尹汉东； 闫淑青；

【Author】 Min Hong;Jin Zhu;Handong Yin;Shuqing Yan;School of Chemistry and Chemical Engineering, Liaocheng University;State Key Laboratory of Coordination Chemistry, Nanjing University;

【机构】 聊城大学化学化工学院； 南京大学配位化学国家重点实验室；

【摘要】 <正>在分子生物学和生物化学领域,以及疾病的诊断,DNA杂交已成为一个有力的工具,用于检测、分离以及分析具体的寡核苷酸序列[1]。MALDI-TOF MS技术是一种有效的诊断工具,可以实现对蛋白质和核酸等生物大分子的分析[2]。本文中,我们报道了设计和构筑一种基于金纳米粒子的多孔纳米结构,将其用于MALDI-TOF MS对表面组装的DNA杂交体系的分析。为了产生纳米多孔更多还原

【Abstract】 In the fields of molecular biology and biochemistry, as well as in the practical diagnosis of diseases, DNA hybridization has become a powerful tool for the isolation, detection and analysis of specific oligonucleotide sequences.1 MALDI-TOF MS has been a diagnostic tool developed primarily for the analysis of biopolymers including proteins and nucleic acids.2 In this paper, we report the design and construction of a gold nanoparticle-assembled porous nanostructure for the effective MALDI-TOF MS analysis of a surface-confined DNA hybridization system. The nanoporous material is derived from layer-by-layer assemblies of negatively charged gold nanoparticles and positively polymers on an underlying silicon wafer, which provides large surface area and good biocompatibility for biomolecules surface analysis. The substrate can be used for the immobilization of thiolated oligonucleotides on gold nanoparticles. The morphology of the porous material was carefully examined with scanning electron microscopy(SEM)(Figure 1). The immobilization of three-strand hybridized sandwich double-strand DNA(Capture DNA-1 + Linker NDA-2 + End DNA-3) on the gold nanoparticle-based interfaces was studied with MALDI-TOF MS. From Figure 2 we can see that the spectrum showed two clear peaks, with a stronger on(4053.8) corresponding to [DNA-3 + K]+, and the weaker one(8405.2) assigned as [DNA-2 + H]+.更多还原