【作者】 罗芳； 林振宇； 邱彬； 陈国南；

【Author】 Fang Luo;Zhenyu Lin;Bin Qiu;Guonan Chen;Ministry of Education Key Laboratory of Analysis and Detection for Food Safety,Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety,Fuzhou University;

【机构】 食品安全分析与检测教育部重点实验室,福建食品安全分析与检测重点实验室,福州大学化学系；

【摘要】 <正>电致化学发光是在化学发光的基础上发展起来的一种分析方法,它结合了化学发光与电化学的优点,具有灵敏高、选择好、易于实现连续自动分析等特点[1]。ATP是生物体内的重要能源物质,是体内能量代谢和转换的中心。因此对ATP的检测不仅具有一定的研究意义还有重要的临床应用价值。为了实现对ATP的高灵敏检测,我们设计并实现了一种基于ATP更多还原

【Abstract】 A novel signal-on electrochemiluminescent(ECL) biosensor based on adenosine triphosphate(ATP)-induced recombination of its split aptamer chips was presented. A 27-base anti-ATP aptamer had been split into two chips: ss DNA1 and ss DNA2. The former had been immobilized onto the gold electrode surface via Au-thiol interaction and worked as the capture probe, whilst the latter one had been functionalized with ECL reagent(tris(2,2’-bipyridyl) ruthenium(II)-doped silica nanoparticles(Ru-Si NPs)) and worked as the detection probe. In the present of ATP, two chips will recombinate together to form an ATP aptamer and results in the immobilization of the Ru-Si NPs on the electrode surface. The ECL intensity from the electrode is then significantly enhanced. It is found that the ECL intensities had a linear relationship with the logarithm of ATP concentration from 1.0×10-12 to 5.0×10-10 mol/L. The sensitivity is sufficiently high for most practical samples suggesting that it is a promising and powerful method for molecules detection.更多还原