【作者】 郭亚青； 詹天荣； 孙伟； 焦奎；

【Author】 Yaqing Guo;Tianrong Zhan;Wei Sun;Kui Jiao;College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology;

【机构】 青岛科技大学化学与分子工程学院；

【摘要】 <正>以正己基吡啶六氟磷酸盐为修饰剂制备碳离子液体电极(CILE),进一步将肌红蛋白(Mb)与Mg2Al水滑石(LDH)和离子液体1-乙酸基-3-甲基咪唑四氟硼酸盐(CMMIMBF4)的复合材料涂布在CILE的表面制备了相应的蛋白质修饰电极(LDH-IL-Mb/CILE)。紫外可见光谱表明Mb在复合膜内保持原有构象,电更多还原

【Abstract】 A new electrochemical biosensor was constructed by immobilization of myoglobin(Mb) on a Mg2Al-layered double hydroxide(LDH) and ionic liquid 1-carboxyl-methyl-3-methylimidazolium tetrafluoroborate(CMMIMBF4) modified carbon ionic liquid electrode(CILE), which was prepared by using N-hexylpyridinium hexafluorophosphate(HPPF6) as the modifier. The resulting LDH-IL-Mb film was characterized by Ultraviolet-Visible(UV-Vis) spectrophotometer, which indicated that Mb retained its native structure in the composite film. A pair of well-defined and quasi-reversible cyclic voltammetric peaks appeared in p H 7.0 phosphate buffer solution with the formal potential-0.204 V(vs. SCE), which was typical electrochemical response of Mb Fe(III)/Fe(II) redox couple. The results were attributed to the synergistic effects of LDH and IL in the composite film, which promoted the electron transfer rate of Mb with the underlying electrode. The electrocatalytic behavior of the modified electrode to the reduction of trichloroacetic acid(TCA) was carefully investigated.更多还原