【作者】 肖芳； 覃益民； 刘幽燕；

【Author】 XIAO Fang;QIN Yi-ming;LIU You-yan;School of Chemistry and Chemical Engineering ,Guangxi University;

【机构】 广西大学化学化工学院； 广西生物炼制重点实验室；

【摘要】 为了提高纤维素酶降解纤维素底物的能力,获得可协同纤维素酶降解纤维素底物的增效蛋白,从土壤中筛选可协同纤维素酶的放线菌。采用稀释涂布法分离,重铬酸钾溶液加入土壤悬浮液中抑制细菌生长进行筛选;根据放线菌菌落形态、培养特征、生理生化特进行初步选择和鉴定。通过分离筛选得到3株放线菌M1、M2、M3,3株放线菌胞内蛋白均能协同纤维素酶降解纤维素底物,产物还原糖的生成量是纤维素酶单独作用的2.5-3.5倍,而其本身不能生成可检测还原糖。更多还原

【Abstract】 In order to improve the ability of cellulase enzymes to degrade cellulose substrates, obtained cellulases synergistically to degrade cellulose substrate synergistic protein from the soil filter cellulases synergistically actinomycetes. Dilution using separate coating method, a suspension of potassium dichromate solution added to the soil to inhibit bacterial growth were screened; according to actinomycetes colony morphology, cultural characteristics, physiological and biochemical special preliminary selection and identification. By separating screened three actinomycetes M1, M2, M3,3 actinomycetes within the extracellular protein can co-cellulases degrade cellulose substrate, the amount of reducing sugar is a cellulase enzyme product acting alone 2.5 to 3.5 times, which itself can’t generate a detectable reducing sugar.更多还原