【作者】 陈操； 孟祥盈； 白敏冬； 程超； 马彬； 李嘉欣；

【Author】 CHEN Cao;MENG Xiangying;BAI Mindong;CHENG Chao;MA Bin;LI Jiaxin;Dalian Maritime University,Institute of Environmental Engineering;College of Environment and Ecology,Xiamen University;

【机构】 大连海事大学环境工程研究所； 厦门大学环境与生态学院；

【摘要】 研究目的:使用羟基自由基压载水处理系统,对海洋危害性赤潮藻类,米氏凯伦藻进行杀灭研究,为揭示·OH致死有害微生物的生物学机制提供依据。研究方法:利用大气压下强电场电离放电技术,制备高浓度羟基溶液,利用羟基的氧化性、高效性、无选择性的特点,对压载水中入侵赤潮藻类米氏凯伦藻进行杀灭。并利用直接显微技术,荧光染色和SEM电镜对羟基处理前后藻细胞进行形态学观察,同时检测细胞内脂质过氧化终产物MDA含量和抗氧化酶系酶活力的变化情况。研究结果:藻的致死效率跟TRO浓度成正比,TRO浓度越高,藻致死效果越明显。当管路中注入足够浓度的羟基溶液时,能够非常有效的杀灭米氏凯伦藻。直接显微技术,荧光染色和SEM电镜的结果也同样证明·OH处理后的藻细胞有明显的形变,萎缩和破碎现象的发生。随着TRO浓度的升高,MDA含量持续增加,而抗氧化酶酶活力呈现先升高后降低的趋势,这一系列变化说明细胞发生了脂质过氧化效应。研究结论:通过大气压强电场放电的方法,规模化制备羟基自由基,能够快速、高效、致死米氏凯伦藻。处理前后藻形态学比较,观察结果显示,·OH的注入打破了,藻细胞壁和细胞膜的屏障作用,使细胞整体通透性增加,细胞内物质转移到了细胞外,细胞由于缺少某些生存所必需的细胞器和胞内物质而失活。通过对细胞内SOD、CAT、POD酶活力以及MDA含量的检测进一步证明了·OH是通过攻击细胞膜上的不饱和脂肪酸,诱发脂质过氧化作用的发生,从而引起细胞结构损伤,最终导致细胞死亡。更多还原

【Abstract】 Objective:Study on using hydroxyl radicals ballast water treatment system to kill the Marine harmful red tide algae(Mikimoto).The result will provide experiment base for ·OH biological effect mechanism research.Methods:With physical methods of strong electric field ionization discharge at atmospheric pressure,forming a high concentration of ·OH solution which was used to kill Mikimoto in ballast water.The morphologic variation were observed by microscopy,fluorescent staining and Electron microscopy(SEM).Meanwhile,detection of intracellular lipid peroxidation products malondialdehyde(MDA) content and antioxidant enzyme system changes of the enzyme activity.Results:Efficiency of algal death is proportional to the concentration of TRO.With the high concentration of TRO was injected into the pipeline,algaes had been killed effectively.Direct microscopy,fluorescent staining and SEM results also prove that after treated by ·OH,The deformation,atrophy and broken of algal cells were obvious observed.The content of MDA increasedsteadily as the increase of concentration of TRO.At the same time,the trend of antioxidant enzymes activity were consistently increased first and then decreased.The series of changes directly and clearly reflect that cell lipid peroxidation was produced.Conclusion:By the method of atmospheric pressure electric discharge preparation of hydroxyl radicals could rapidly,efficiently kill Mikimoto.Algae morphological comparison before and after the treated by ·OH.The observation results show that · OH could broke the barrier function cell wall and cell membrane of algea,increased cell membrane penetrability,Transported intracellular substances out of the cell,lead to Cell inactivation ultimately.Through the detection of intracellular SOD,CAT,POD activity and MDA content further proved that ·OH could attack on the cell membrane unsaturated fatty acids,which induced the occurrence of lipid peroxidation,caused cell structural damage,eventually lead to cell death.更多还原