抗病促生枯草芽孢杆菌B29在黄瓜根际的定殖研究

【作者】 李晶； 张先成； 赵晓宇； 王玉霞； 孟利强； 张云湖； 张淑梅；

【机构】 黑龙江省科学院微生物研究所；

【摘要】 采用利福平抗性诱导方法对枯草芽孢杆菌B29菌株进行标记,研究该生防菌在黄瓜幼苗根际的定殖规律。结果在温室条件下,通过浸种、灌根或两种处理方式相结合,枯草芽孢杆菌B29菌株均可在黄瓜幼苗根内、茎基部和根际土壤中很好地定殖。浸种和灌根相结合的处理方式利于生防菌的定殖。该菌株较强的定殖能力使其可以充分发挥空间、营养竞争和抑制病菌的作用,从而表现较好的防治效果。更多还原

【Abstract】 In order to investigate the colonization of Bacillus subtilis B29 in cucumber rhizosphere,the strain was marked by using the method of antibiotic(rifampicin)resistance.The results showed that strain B29 colonized in the root,basal stem and rhizosphere soil of cucumber seedling after soaking seed or watering root treatment or combination treatment in greenhouse or in field.It was better for colonization by the treatment first soaking seed then watering root.The strong ability of Bacillus subtilis B29 to colonize helped it to compete site and nutrients with pathogens and then to inhibit pathogens effectively.更多还原