基于16S rRNA基因克隆文库对花生根际铁载体产生菌的分析

【作者】 王文铜； 杜秉海； 姚良同； 王善林； 陈修红； 丁延芹；

【机构】 山东农业大学生命科学学院 山东农业微生物重点实验室；

【摘要】 [目的]通过结合16S rDNA克隆文库,对花生根际铁载体高产菌进行系统发育分析。[方法]通过CAS(Chrome Azurol S)检测、ARDRA(扩增性rDNA限制性酶切片段分析)等方法从花生根际土样中筛选到铁载体高产菌,分析其16S rDNA序列,并针对花生土壤样品构建了三个16S rDNA克隆文库,结合两者对花生根际铁载体高产菌进行遗传多样性和系统发育分析。[结果]35株产铁载体能力比较强的细菌通过分析,所有菌株分为7个组。说明分离到的花生根际土壤铁载体菌存在较大的遗传多样性,其中Firmicutes、γ-proteobacteria和Bacteroidetes属于优势菌。对三个花生土样构建的16S rDNA克隆文库分析说明Firmicutes和Acidobacteria属于优势菌,但是,Acidobacteria中却没有产铁载体能力较强的菌。另外,虽然Bacteroidetes中,产铁载体能力强的菌比例比较大,但是它受到施肥情况的影响明显。[结论]在16S rDNA克隆文库的基础上对铁载体高产菌的系统发育研究,可以有效的提高研究铁载体高产菌效率,并对铁载体高产菌的系统发育地位作出了研究。更多还原

【Abstract】 [Objective]Make phylogeny analysis of bacteria producing siderophores high yield which come from rhizosphere of peanut combining 16 S rDNA cloning libraries.[Methods]By means of Chrome Azurol S,Amplified Ribosomal DNA Restriction Analysis(ARDRA),isolated bacteria producing siderophores high yield from peanut rhizosphere soils,analysed their 16 s rDNA sequences,and structured 16 S rDNA cloning libraries for the samples.Combining the both make the phylogeny and genetic variety analysis of the bacteria producing siderophores high yield.[Results]By analysis,All the thirty-five bacteria producing siderophores high yield could be classified into 8 groups,which declared the genetic diversity of the siderophore strains isolated from peanut rhizospheric soils is high.The predominant bacteria were Firmicutes,γ-proteobacteria and Bacteroidetes.By analyzed three 16 S rDNA cloning libraries indicated Firmicutes and Acidobacteria belong to predominant bacteria.But there were no bacteria producing siderophores high yield found in Acidobacteria.Though bacteria producing siderophores high yield held a high specific gravity in Bacteroidetes,they were influenced obviously by fertilizer,[Conclusion]Making phylogeny analysis of bacteria producing siderophores high yield based on 16 S rDNA cloning libraries can improve efficiency of the research for bacteria producing siderophores high yield availably,and discussed the status of the bacteria in the phylogeny analysis.更多还原