【作者】 张晓燕； 温海深； 何峰； 李吉方； 王海亮； 司玉凤； 任源远；

【Author】 ZHANG Xiaoyan;WEN Haishen;LI Jifang;HE Feng;WANG Hailiang;SI Yufeng;REN Yuanyuan;Fisheries College, Ocean University of China;

【机构】 中国海洋大学水产学院；

【摘要】 盐度是一种重要的养殖环境因子,能够影响鱼类生长、繁育和代谢等多种生理过程。花鲈是我国海水养殖重要经济鱼类之一,具有广盐性的特性。为了探究花鲈对不同盐度的分子生理适应机制,本论文采用高通量测序对生长在两个不同盐度下的花鲈肝组织转录组进行了测定。测序结果共产生了197550条unigenes,采用DESeq法筛选出了455个差异基因,其中上调表达基因184个,下调表达基因271个。用生物信息学技术对转录组进行基因功能注释和分析,GO富集分析结果显示,基因主要富集在细胞过程、信号转导、细胞定位和离子转运等生物过程。KEGG富集分析表明显著变化的基因发生在信号转导、细胞免疫、内分泌等过程。筛选了溶质转运蛋白家族6成员20(slc6a20)、slc39a4、内向整流性钾通道16、水通道蛋白3、白细胞介素8、骨生成蛋白等基因进行了实时定量验证。本研究为花鲈的盐度适应性研究提供了一系列候选基因,为进一步阐明这些关键基因在渗透调控中的生理功能奠定了基础。更多还原

【Abstract】 Salinity can influence physiological and metabolic activity, reproduction, growth and development of marine fishes. Spotted sea bass(Lateolabrax maculatus), as a euryhaline teleost, is widely distributed across China’s coastline. To investigate salinity adaptation mechanism, we experimentally conducted salinity conditions for two groups of spotted sea bass and measured differences in RNA transcriptome between them. The present study yielded 197550 unigenes.Through Illumina sequencing, 184 up-regulated genes and 271 down-regulated geneswere reported.As determined by GO analysis and KEGG pathway mapping, functional annotation ofthe genes covered diverse biological functions and processes.The genes that changed expression significantly werehighly represented cellular processes, signal transduction, cell positioning and transfer activity according to GO annotation.The KEGG results highlighted genes related to osmoregulation, Signal transduction, cellular immunity, endocrine and so on.We screened slc6a20、slc39a4、inward rectifier potassium channel 16-like、aquaporin3、interleukin-8-like、bone morphogenetic protein 3B-like gene to do real-time PCR validation.Thestudy offered a number of candidate genesof salinityadaptation for spotted sea bass and will facilitate research into extensive transcriptional plasticity to find underlying physiological adaptations todifferent salinities for marine fish.更多还原