【作者】 韩明明； 卢建国； 孙效文；

【机构】 中国水产科学研究院黑龙江水产研究所； 上海海洋大学水产与生命学院；

【摘要】 采用RT-PCR技术,克隆出黄颡鱼N-乙酰基转移酶9(N-acetyltransferase 9)基因c DNA全长序列,应用real-time q PCR法对雌雄个体不同组织中N-acetyltransferase 9 m RNA的表达进行检测。序列分析表明,N-乙酰基转移酶9,基因c DNA全长为1253bp,包括584的非编码区(untranslation region,UTR),699bp(open reading frame,ORF)的开放阅读框。N-acetyltransferase 9是由222个氨基酸组成。型号肽分析、进化树构建、采用相对荧光定量技术检以及其所编码蛋白的三级结构预测。更多还原

【Abstract】 RT-PCR technique, cloned Pelteobagrus fulvidraco N- acetyltransferase gene full-length c DNA sequences.Application of real-time PCR method for the expression of male and female individuals in different tissues N-acetyltransferase 9 m RNA were detected.Sequence analysis showed that, Nacetyltransferase 9 full-length c DNA is 1253 bp, including 584 non-coding region,699 bp open reading frame. N-acetyltransferase 9 is composed of 222 amino acids.Type peptide analysis, phylogenetic tree constructed using tertiary structure prediction relative fluorescence detection。N- acetyltransferase 9 in the tertiary structure prediction encoded proteins.更多还原